Yang Rongchang, Brice Belinda, Elloit Aileen, Ryan Una
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia, 6076, Australia.
Exp Parasitol. 2016 Jul;166:124-30. doi: 10.1016/j.exppara.2016.04.009. Epub 2016 Apr 12.
An Eimeria species is described from a domestic pigeon (Columba livia domestica). Sporulated oocysts (n = 35) were subspherical, with a smooth bi-layered oocyst wall (1.0 μm thick). Oocysts measured 20.2 × 16.1 (22.0-18.9 × 15.7-18.9) μm, oocyst length/width (L/W) ratio, 1.38. Oocyst residuum and a polar granule were present. The micropyle was absent. Sporocysts are elongate-ovoid, 13.0 × 6.1 (14.5-12.5 × 5.5-7.0) μm, sporocyst L/W ratio, 2.13 (2.0-2.2), sporocyst residuum was present, composed of numerous granules in a spherical or ovoid mass. Each sporocyst contained 2 banana-shaped sporozoites, 12.3 × 3.5 (11.8-13.0 × 3.3-3.6) μm. A spherical-ellipsoid posterior refractile body was found in the sporozoites. A nucleus was located immediately anterior to the posterior refractile body. Molecular analysis was conducted at three loci; the 18S and 28S ribosomal RNA genes and the mitochondrial cytochrome oxidase gene (COI). At the 18S locus, the new isolate shared 98.0% genetic similarity with three Isospora isolates from Japan from the domestic pigeon (Columba livia domestica). At the 28S locus, it grouped separately and shared 92.4% and 92.5% genetic similarity with Isospora anthochaerae (KF766053) from a red wattlebird (Anthochaera carunculata) from Australia and an Isospora sp. (MS-2003 - AY283845) from a Himalayan grey-headed bullfinch (Pyrrhula erythaca) respectively. At COI locus, this new isolate was in a separate clade and shared 95.6% and 90.0% similarity respectively with Eimeria tiliquae n. sp. from a shingleback skink in Australia and an Eimeria sp. from a common pheasant (Phasianus colchicus) from America. Based on the morphological data, this isolate is most similar to Eimeria labbeana. As no molecular data for E. labbeana is available and previous morphological data is incomplete, we refer to the current isolate as E. labbeana-like.
从一只家鸽（Columba livia domestica）中描述了一种艾美耳球虫。孢子化卵囊（n = 35）呈亚球形，卵囊壁光滑，双层结构（厚1.0μm）。卵囊大小为20.2×16.1（22.0 - 18.9×15.7 - 18.9）μm，卵囊长宽比（L/W）为1.38。有卵囊残体和一个极粒。无微孔。孢子囊呈长椭圆形，13.0×6.1（14.5 - 12.5×5.5 - 7.0）μm，孢子囊长宽比为2.13（2.0 - 2.2），有孢子囊残体，由许多颗粒组成球形或椭圆形团块。每个孢子囊含2个香蕉形子孢子，12.3×3.5（11.8 - 13.0×3.3 - 3.6）μm。在子孢子中发现一个球形 - 椭圆形后折光体。细胞核位于后折光体正前方。在三个基因位点进行了分子分析；18S和28S核糖体RNA基因以及线粒体细胞色素氧化酶基因（COI）。在18S位点，新分离株与来自日本家鸽（Columba livia domestica）的三种等孢球虫分离株的遗传相似性为98.0%。在28S位点，它单独聚类，与来自澳大利亚红颈吸蜜鸟（Anthochaera carunculata）的等孢球虫（KF766053）以及来自喜马拉雅灰头朱雀（Pyrrhula erythaca）的一种等孢球虫（MS - 2003 - AY283845）的遗传相似性分别为92.4%和92.5%。在COI位点，这个新分离株处于一个单独的分支中，与来自澳大利亚松果蜥的蒂氏艾美耳球虫新种以及来自美国环颈雉（Phasianus colchicus）的一种艾美耳球虫的相似性分别为95.6%和90.0%。基于形态学数据，该分离株与拉贝艾美耳球虫最相似。由于没有拉贝艾美耳球虫的分子数据且之前的形态学数据不完整，我们将当前分离株称为类拉贝艾美耳球虫。
