来自西澳大利亚一只家养金丝雀（Serinus canaria forma domestica）（雀形目：燕雀科）的新种等孢球虫（Isospora serinuse n. sp.）（顶复门：艾美耳科）
Isospora serinuse n. sp. (Apicomplexa: Eimeriidae) from a domestic canary (Serinus canaria forma domestica) (Passeriformes: Fringillidae) in Western Australia.
作者信息
Yang Rongchang, Brice Belinda, Elliot Aileen, Ryan Una
机构信息
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia 6076, Australia.
出版信息
Exp Parasitol. 2015 Dec;159:59-66. doi: 10.1016/j.exppara.2015.08.020. Epub 2015 Aug 29.
A new species, Isospora serinuse n. sp., (Apicomplexa:Eimeriidae) is described from a single domestic canary (Serinus canaria forma domestica) (subspecies S. c. domestica) in Western Australia. Sporulated oocysts of Isospora serinuse n. sp. are spherical or subspherical, 25.5 (24.4-27.0) × 23.5 (22.0-24.8) μm, with a shape index (length/width) of 1.09; and a smooth bilayered oocyst wall, 1.2 μm thick (outer layer 0.9 μm, inner 0.3 μm). A polar granule is present, but a micropyle and oocyst residuum are absent. The sporocysts are lemon-shaped, 18.9 (17.8-20.2) × 11.8 (10.6-13.0) μm, with a shape index of 1.6. Stieda and substieda bodies are present, the Stieda body being a small crescent shape and the substieda being indistinct. Each sporocyst with four vermiform sporozoites arranged head to tail. A sporocyst residuum is present and composed of numerous granules of different sizes that are scattered among the sporozoites. Morphologically, the oocysts of Isospora serinuse n. sp. were different from those of all known valid Isospora spp. Molecular analysis was conducted at 3 loci: the 18S and 28S ribosomal RNA and two separate regions of subunit I of the mitochondrial cytochrome oxidase (COI) gene (designated COIa and COIb). At the 18S locus, Isospora serinuse n. sp. exhibited 97.5% similarity to Isospora sp. Tokyo from a domestic pigeon (Columba livia domestica) in Japan. At the 28S locus, I. serinuse n. sp. exhibited 94.9% similarity to Isospora anthochaerae n. sp. from a red wattlebird (Anthochaera carunculata) in Australia. At the COIa locus, I. serinuse n. sp. exhibited 95.7% similarity to Isospora sospora sp. ex Apodemus flavicollis from a yellow-necked mouse and Isospora gryphoni from an American goldfinch (Carduelis tristis) respectively. At the COIb locus, I. serinuse n. sp. exhibited 96.7% similarity to an Isospora (iSAT4) from a European pied flycatcher (Ficedula hypoleuca). Based on morphological and molecular data, this isolate is a new species of Isospora, which is named Isospora serinuse n. sp. after its host, the domestic canary (S. canaria forma domestica).
在西澳大利亚，从一只家养金丝雀（Serinus canaria forma domestica）（亚种S. c. domestica）中发现了一个新物种，即家金丝雀等孢球虫（Isospora serinuse n. sp.）（顶复门：艾美耳科）。家金丝雀等孢球虫的孢子化卵囊呈球形或亚球形，大小为25.5（24.4 - 27.0）×23.5（22.0 - 24.8）μm，形状指数（长/宽）为1.09；卵囊壁光滑，双层，厚1.2μm（外层0.9μm，内层0.3μm）。有一个极粒，但无微孔和卵囊残体。孢子囊呈柠檬形，大小为18.9（17.8 - 20.2）×11.8（10.6 - 13.0）μm，形状指数为1.6。有斯氏体和亚斯氏体，斯氏体呈小新月形，亚斯氏体不明显。每个孢子囊内有四个蠕虫状子孢子，头对头排列。有孢子囊残体，由许多大小不同的颗粒组成，散布在子孢子之间。形态上，家金丝雀等孢球虫的卵囊与所有已知有效的等孢球虫物种的卵囊不同。在三个基因座进行了分子分析：18S和28S核糖体RNA以及线粒体细胞色素氧化酶（COI）基因亚基I的两个独立区域（分别命名为COIa和COIb）。在18S基因座，家金丝雀等孢球虫与来自日本一只家鸽（Columba livia domestica）的东京等孢球虫（Isospora sp. Tokyo）相似度为97.5%。在28S基因座，家金丝雀等孢球虫与来自澳大利亚一只红颈吸蜜鸟（Anthochaera carunculata）的红颈吸蜜鸟等孢球虫（Isospora anthochaerae n. sp.）相似度为94.9%。在COIa基因座，家金丝雀等孢球虫分别与来自黄颈姬鼠的黄颈姬鼠等孢球虫（Isospora sospora sp. ex Apodemus flavicollis）和来自美洲金翅雀（Carduelis tristis）的灰氏等孢球虫（Isospora gryphoni）相似度为95.7%。在COIb基因座，家金丝雀等孢球虫与来自欧洲斑姬鹟（Ficedula hypoleuca）的一种等孢球虫（iSAT4）相似度为96.7%。基于形态和分子数据，该分离株是等孢球虫的一个新物种，以其宿主家养金丝雀（S. canaria forma domestica）命名为家金丝雀等孢球虫。
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