Li Xiang, Lee Yun Jung, Kim Hye Yoom, Tan Rui, Park Min Cheol, Kang Dae Gill, Lee Ho Sub
* College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk 540-749, Republic of Korea.
† Body-Fluid Research Center, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk 570-749, Republic of Korea.
Am J Chin Med. 2016;44(2):305-20. doi: 10.1142/S0192415X1650018X.
We have reported that ethanol extracts of the root from Scutellaria baicalensis Georgi (ESB) relax cavernous smooth muscles via the NO/cGMP system and Ca[Formula: see text]-sensitive K[Formula: see text] channels in the rabbit corpus cavernosum. In the present study, erectile function was assessed by intracavernous pressure (ICP) and mean arterial pressure (MAP) during electrical stimulation of the cavernous nerve. The ICP/MAP ratio was dose-dependently increased by the treatment of ESB in normal SD rats ([Formula: see text]). To investigate the beneficial effect of ESB on erectile dysfunction in a diabetic animal model, male SD rats were injected with streptozotocin (60[Formula: see text]mg/kg) and then 300[Formula: see text]mg/kg/day ESB was administered daily for eight weeks. In our in vivo study, administration of ESB in STZ rats significantly increased the ICP, ICP/MAP ratio, area under the curve (AUC), as well as the cavernous cGMP levels. Morphometric analyses showed that ESB administration increased both smooth muscle volume and the regular arrangement of collagen fibers compared to the STZ group. The protein expression levels of endothelial nitric oxide synthase (eNOS) and SM [Formula: see text]-actin from penile tissues were also significantly increased in the ESB-treated rats. Taken together, these results suggest that ESB ameliorates penile erectile dysfunction via the activation of the NO/cGMP pathways of the penile corpus cavernosum in a streptozotocin-induced diabetic rat model.
我们曾报道，黄芩根乙醇提取物（ESB）可通过兔海绵体中的NO/cGMP系统和钙敏感性钾通道使海绵体平滑肌松弛。在本研究中，通过在电刺激海绵体神经期间测量海绵体内压（ICP）和平均动脉压（MAP）来评估勃起功能。在正常SD大鼠中，ESB处理可使ICP/MAP比值呈剂量依赖性增加（[公式：见原文]）。为了研究ESB对糖尿病动物模型勃起功能障碍的有益作用，给雄性SD大鼠注射链脲佐菌素（60[公式：见原文]mg/kg），然后每天给予300[公式：见原文]mg/kg的ESB，持续八周。在我们的体内研究中，给STZ大鼠施用ESB可显著提高ICP、ICP/MAP比值、曲线下面积（AUC）以及海绵体cGMP水平。形态计量学分析表明，与STZ组相比，施用ESB可增加平滑肌体积并使胶原纤维排列更规则。在接受ESB处理的大鼠中，阴茎组织中内皮型一氧化氮合酶（eNOS）和SM[公式：见原文]-肌动蛋白的蛋白表达水平也显著增加。综上所述，这些结果表明，在链脲佐菌素诱导的糖尿病大鼠模型中，ESB通过激活阴茎海绵体的NO/cGMP途径改善阴茎勃起功能障碍。
