Tan Rui, Lee Yun Jung, Cho Kyung Woo, Kang Dae Gill, Lee Ho Sub
College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea.
Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk, 570-749, Republic of Korea.
Chin J Integr Med. 2018 Jun;24(6):448-454. doi: 10.1007/s11655-017-2920-z. Epub 2018 Jan 15.
To investigate whether the methanol extract of Berberis amurensis Rupr. (BAR) augments penile erection using in vitro and in vivo experiments.
The ex vivo study used corpus cavernosum strips prepared from adult male New Zealand White rabbits. In in vivo studies for intracavernous pressure (ICP), blood pressure, mean arterial pressure (MAP), and increase of peak ICP were continuously monitored during electrical stimulation of Sprague-Dawley rats.
Preconstricted with phenylephrine (PE) in isolated endotheliumintact rabbit corus cavernosum, BAR relaxed penile smooth muscle in a dose-dependent manner, which was inhibited by pretreatment with NG-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, and H-[1,2,4]-oxadiazole-[4,3-α]-quinoxalin-1-one, a soluble guanylyl cclase inhibitor. BAR significantly relaxed penile smooth muscles dose-dependently in ex vivo, and this was inhibited by pretreatment with L-NAME H-[1,2,4]-oxadiazole-[4,3-α]-quinoxalin-1-one. BAR-induced relaxation was significantly attenuated by pretreatment with tetraethylammonium (TEA, P<0.01), a nonselective K channel blocker, 4-aminopyridine (4-AP, P<0.01), a voltage-dependent K channel blocker, and charybdotoxin (P<0.01), a large and intermediate conductance Ca sensitive-K channel blocker, respectively. BAR induced an increase in peak ICP, ICP/MAP ratio and area under the curve dose dependently.
BAR augments penile erection via the nitric oxide/cyclic guanosine monophosphate system and Ca sensitive-K (BK and IK) channels in the corpus cavernosum.
通过体外和体内实验研究刺黄柏甲醇提取物（BAR）是否能增强阴茎勃起功能。
体外研究采用成年雄性新西兰白兔制备的海绵体条。在体内研究中，对Sprague-Dawley大鼠进行电刺激时，持续监测海绵体内压（ICP）、血压、平均动脉压（MAP）以及峰值ICP的增加情况。
在离体的内皮完整的兔海绵体中，用去氧肾上腺素（PE）预收缩后，BAR以剂量依赖的方式松弛阴茎平滑肌，一氧化氮合酶抑制剂NG-硝基-L-精氨酸甲酯（L-NAME）和可溶性鸟苷酸环化酶抑制剂H-[1,2,4]-恶二唑-[4,3-α]-喹喔啉-1-酮预处理可抑制这种作用。在体外，BAR能剂量依赖性地显著松弛阴茎平滑肌，且L-NAME和H-[1,2,4]-恶二唑-[4,3-α]-喹喔啉-1-酮预处理可抑制该作用。分别用非选择性钾通道阻滞剂四乙铵（TEA，P<0.01）、电压依赖性钾通道阻滞剂4-氨基吡啶（4-AP，P<0.01）和大电导及中电导钙敏感性钾通道阻滞剂蝎毒素（P<0.01）预处理后，BAR诱导的松弛作用明显减弱。BAR能剂量依赖性地诱导峰值ICP、ICP/MAP比值和曲线下面积增加。
BAR通过海绵体中的一氧化氮/环磷酸鸟苷系统和钙敏感性钾通道（大电导钾通道和中电导钾通道）增强阴茎勃起功能。
