Minoura N, Aiba S, Fujiwara Y, Koshizaki N, Imai Y
Industrial Products Research Institute, Ibaraki, Japan.
J Biomed Mater Res. 1989 Feb;23(2):267-79. doi: 10.1002/jbm.820230210.
Random copolypeptides and block copolypeptides were synthesized, and an interaction between these polypeptide membranes and the cells was studied by a cell culture method (cell line, Ca. 9.22). In random copolypeptides composed of gamma-methyl L-glutamate and gamma-benzyl L-glutamate, cell attachment and cell growth depended on the monomer composition, and showed a maximum at around 70 mole % of benzyl glutamate. Block copolypeptide composed of L-methionine and oxyethylene exhibited low cell attachment and cell growth even at 10 mole % of oxyethylene content, compared to L-methionine homopolymer. ESCA study of the membrane suggested this result to be due to concentration of the poly(oxyethylene) block chain of the polymer on the surface of the membrane. Block copolypeptide composed of N5-(3-hydroxypropyl) L-glutamine and L-leucine exhibited low cell attachment and cell growth, while the corresponding random copolypeptide exhibited high cell attachment and cell growth. This difference is attributable to the microheterophase structure with the hydrophilic domains embedded in the hydrophobic matrix in the block copolypeptide membrane.
合成了无规共多肽和嵌段共多肽，并采用细胞培养法（细胞系，Ca. 9.22）研究了这些多肽膜与细胞之间的相互作用。在由γ-甲基-L-谷氨酸和γ-苄基-L-谷氨酸组成的无规共多肽中，细胞附着和细胞生长取决于单体组成，在苄基谷氨酸含量约为70摩尔%时达到最大值。与L-甲硫氨酸均聚物相比，由L-甲硫氨酸和氧化乙烯组成的嵌段共多肽即使在氧化乙烯含量为10摩尔%时也表现出低细胞附着和低细胞生长。对该膜的电子能谱化学分析（ESCA）表明，该结果是由于聚合物的聚（氧化乙烯）嵌段链在膜表面富集所致。由N5-（3-羟丙基）-L-谷氨酰胺和L-亮氨酸组成的嵌段共多肽表现出低细胞附着和低细胞生长，而相应的无规共多肽则表现出高细胞附着和高细胞生长。这种差异归因于嵌段共多肽膜中亲水域嵌入疏水基质的微相异质性结构。
