Kang Hyeok-Joong, Lee In Kyu, Piao Min-Yu, Kwak Chae-Won, Gu Min Jeong, Yun Cheol Heui, Kim Hyun-Jin, Ahn Hyeon-Ju, Kim Hee-Bal, Kim Gyeom-Heon, Kim Soo-Ki, Ko Jong-Youl, Ha Jong K, Baik Myunggi
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Institutes of Green Bio Science Technology, Seoul, Republic of Korea.
Anim Sci J. 2017 Jan;88(1):140-148. doi: 10.1111/asj.12604. Epub 2016 Apr 18.
This study examined the effects of road transportation on metabolic and immunological responses in dairy heifers. Twenty Holstein heifers in early pregnancy were divided into non-transported (NT; n = 7) and transported (T; n = 13) groups. Blood was collected before transportation (BT), immediately after transportation for 100 km (T1) and 200 km (T2), and 24 h after transportation (AT). The T heifers had higher (P < 0.05) blood cortisol and non-esterified fatty acid concentrations after T1 and T2 than did NT heifers. By contrast, the T heifers had lower (P < 0.05) serum triglyceride concentrations after T1 and T2 than had the NT heifers. The serum cortisol and triglyceride concentrations returned (P > 0.05) to the BT concentrations at 24 h AT in the T heifers. The granulocyte-to-lymphocyte ratio and the percentage of monocytes were higher (P < 0.05) after T2 in the T heifers than in the NT heifers, suggesting that transportation stress increased the numbers of innate immune cells. T heifers had higher (P < 0.01) plasma haptoglobin concentrations than NT heifers 24 h AT. In conclusion, transportation increased cortisol secretion and was correlated with increased metabolic responses and up-regulation of peripheral innate immune cells in dairy heifers.
本研究考察了公路运输对妊娠初期奶牛代谢和免疫反应的影响。将20头妊娠初期的荷斯坦奶牛分为未运输组（NT；n = 7）和运输组（T；n = 13）。在运输前（BT）、运输100公里后立即（T1）和运输200公里后立即（T2）以及运输后24小时（AT）采集血液。运输组奶牛在T1和T2后的血皮质醇和非酯化脂肪酸浓度高于未运输组奶牛（P < 0.05）。相比之下，运输组奶牛在T1和T2后的血清甘油三酯浓度低于未运输组奶牛（P < 0.05）。运输组奶牛在运输后24小时血清皮质醇和甘油三酯浓度恢复到运输前浓度（P > 0.05）。运输组奶牛在T2后的粒细胞与淋巴细胞比率和单核细胞百分比高于未运输组奶牛（P < 0.05），表明运输应激增加了固有免疫细胞数量。运输组奶牛在运输后24小时的血浆触珠蛋白浓度高于未运输组奶牛（P < 0.01）。总之，运输增加了皮质醇分泌，并与奶牛代谢反应增强和外周固有免疫细胞上调相关。
