Ellis S A, Strachan T, Palmer M S, McMichael A J
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, UK.
J Immunol. 1989 May 1;142(9):3281-5.
We have used Northern blot analysis to detect mRNA from class I HLA genes in the human choriocarcinoma cell line BeWo, which has been previously shown to express an atypical HLA class I molecule, in the absence of HLA A and B. Hybridization was seen with three class I DNA probes, the strongest being seen with the probe pC800, which corresponds to an 800-bp section of the Cw3 gene. We have made cDNA libraries from BeWo cells and screened for positive clones by using class I DNA probes. Of the clones isolated, we determined the complete sequence of one and partial sequence of five shorter clones. They all code for an identical C locus-related product, which does not correspond to published C locus sequences.
我们利用Northern印迹分析来检测人绒毛膜癌细胞系BeWo中I类HLA基因的mRNA，该细胞系先前已被证明在缺乏HLA A和B的情况下表达一种非典型的I类HLA分子。用三种I类DNA探针进行杂交，其中与探针pC800杂交信号最强，该探针对应于Cw3基因的一个800 bp片段。我们从BeWo细胞构建了cDNA文库，并使用I类DNA探针筛选阳性克隆。在分离得到的克隆中，我们确定了一个克隆的完整序列和五个较短克隆的部分序列。它们都编码一种与C位点相关的相同产物，该产物与已发表的C位点序列不对应。
