Mizuno S, Trapani J A, Koller B H, Dupont B, Yang S Y
Laboratory of Biochemical Immunogenetics, Sloan-Kettering Institute, New York 10021.
J Immunol. 1988 Jun 1;140(11):4024-30.
A cDNA clone, designated JTW15, has been isolated and found to encode a novel, divergent class I HLA gene transcript. The clone was identified by its ability to hybridize with a pan-HLA class I nucleotide probe while failing to bind probes specific for the 3' untranslated exons of the HLA-A, -B, and -C genes. JTW15, 1665 nucleotides in length, represents a full length copy of an approximately 1.7-kb mRNA detected in Northern blot analyses. Comparison of the derived amino acid sequence with those of other HLA class I proteins showed JTW15 to be the most divergent HLA class I gene identified so far, the level of sequence homology being lowest for the alpha 1 and alpha 2 domains. The JTW15 gene transcript was found in a broad variety of cell types and was inducible in cells treated with IFN-gamma. JTW15 is the first isolated full length cDNA clone defining a non-HLA-ABC class I gene.
一个名为JTW15的cDNA克隆已被分离出来，发现它编码一种新型的、不同的I类HLA基因转录本。该克隆是通过其与泛I类HLA核苷酸探针杂交的能力而被鉴定出来的，同时它不能与针对HLA - A、- B和- C基因3'非翻译外显子的探针结合。JTW15全长1665个核苷酸，代表了在Northern印迹分析中检测到的一个约1.7kb mRNA的全长拷贝。将推导的氨基酸序列与其他I类HLA蛋白的序列进行比较，结果显示JTW15是迄今为止鉴定出的最不同的I类HLA基因，其α1和α2结构域的序列同源性水平最低。在多种细胞类型中都发现了JTW15基因转录本，并且在用γ干扰素处理的细胞中可被诱导。JTW15是第一个分离出的定义非HLA - ABC I类基因的全长cDNA克隆。
