State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.
Anal Chem. 2016 May 17;88(10):5424-9. doi: 10.1021/acs.analchem.6b00802. Epub 2016 Apr 27.
We report a pseudo-paper microfluidic chip based on patterned photonic nitrocellulose. The photonic nitrocellulose is fabricated using self-assembled monodisperse SiO2 nanoparticles as template. The SiO2 nanoparticles form a photonic crystal having a close-packed hexagonal structure in the microchannels, so the resulting nitrocellulose has a complementary inverse-opal structure. After lamination, a hollow channel is obtained that is partially filled with the photonic nitrocellulose. Owing to the highly ordered photonic structure of the pseudo-paper chip, the flow profile of aqueous solution wicking through the channel is more uniform than conventional paper microfluidic chip. It is also found that the wicking rate of aqueous solution can be easily manipulated by changing the diameter of the self-assembled monodisperse SiO2 nanoparticles, which determines the pore size of the photonic nitrocellulose. The fluorescent enhancement property of the photonic nitrocellulose is used to increase the fluorescent intensity for multiplex detection of two cancer biomarkers. Label-free detection of human immunoglobin G based on the structure color of the photonic nitrocellulose is also demonstrated.
我们报告了一种基于图案化光子纤维素的伪纸微流控芯片。光子纤维素是使用自组装单分散二氧化硅纳米粒子作为模板制备的。二氧化硅纳米粒子在微通道中形成具有密堆积六方结构的光子晶体，因此得到的纤维素具有互补的反蛋白石结构。层压后，获得部分填充有光子纤维素的中空通道。由于伪纸芯片具有高度有序的光子结构，因此水溶液在通道中吸液的流动轮廓比传统的纸张微流控芯片更加均匀。还发现，通过改变自组装单分散二氧化硅纳米粒子的直径可以轻松地控制水溶液的吸液速率，这决定了光子纤维素的孔径。利用光子纤维素的荧光增强特性，可以提高荧光强度，从而实现对两种癌症生物标志物的多重检测。还展示了基于光子纤维素结构颜色的无标记人免疫球蛋白 G 的检测。
