Department of Morphology, Institute of Anatomy and Anthropology, Riga, Latvia.
Indian J Dermatol Venereol Leprol. 2016 May-Jun;82(3):284-91. doi: 10.4103/0378-6323.171652.
Psoriasis vulgaris is an inflammatory skin condition characterized by dramatic biochemical and immunological changes.
The aim of the study was to evaluate antimicrobial response, tissue degeneration reactions and distribution of inflammatory cytokines in untreated psoriatic skin as well as the correlations between these factors and influence on the course of the disease.
We evaluated skin samples obtained from routine punch biopsies in 40 patients with psoriasis vulgaris. All tissue specimens were examined by hematoxylin and eosin staining and immunohistochemistry for human beta defensin 2 (HBD-2), matrix metalloproteinase 2 (MMP-2), tumor necrosis factor-alpha (TNF-alpha), interleukin 6 (IL-6) and IL-8. The staining intensity was semi-quantitatively graded.
Numerous keratinocytes, fibroblasts and macrophages expressed HBD-2 while the number of MMP-2-positive macrophages, fibroblasts and epitheliocytes varied. TNF-alpha-positive cells varied from a few to numerous in each microscopic field. IL-6-positive cells varied from a few to abundant and IL-8-positive cells from numerous to abundant in each field.
This study had a rather small patient number.
Psoriatic skin shows a strong correlative increase in skin antimicrobial proteins and enzymes mediating tissue degeneration suggesting that the skin maintains compensatory mechanisms during persistent remodeling. While individual notable decrease in antimicrobial proteins was observed in some tissue samples, generally the increased human beta defensin associated with psoriasis is likely to be due to an altered immune status. TNF-alpha, IL-6 and IL-8 are common cytokines expressed in psoriatic skin plaques to maintain the inflammatory cycle. HBD-2, MMP-2 and TNF-alpha positively correlate with the severity of psoriasis. Meanwhile, the expression of IL-8 significantly decreases with clinically more severe psoriasis, perhaps making these factors candidate prognostic factors for psoriatic inflammation.
寻常型银屑病是一种炎症性皮肤病，其特征是剧烈的生化和免疫学变化。
本研究旨在评估未经治疗的银屑病皮肤中的抗菌反应、组织退化反应和炎症细胞因子的分布，以及这些因素之间的相关性及其对疾病进程的影响。
我们评估了 40 例寻常型银屑病患者常规打孔活检获得的皮肤样本。所有组织标本均用苏木精和伊红染色和人β防御素 2（HBD-2）、基质金属蛋白酶 2（MMP-2）、肿瘤坏死因子-α（TNF-α）、白细胞介素 6（IL-6）和白细胞介素 8（IL-8）免疫组织化学染色。染色强度进行半定量分级。
大量角质形成细胞、成纤维细胞和巨噬细胞表达 HBD-2，而 MMP-2 阳性巨噬细胞、成纤维细胞和上皮细胞的数量则不同。TNF-α 阳性细胞在每个显微镜视野中从少数到多数不等。IL-6 阳性细胞在每个视野中从少数到丰富，IL-8 阳性细胞从多数到丰富。
本研究的患者数量相对较少。
银屑病皮肤显示出强烈的相关性，即皮肤抗菌蛋白和介导组织退化的酶增加，这表明皮肤在持续重塑过程中维持着代偿机制。虽然在一些组织样本中观察到个别抗菌蛋白明显减少，但一般来说，与银屑病相关的增加的人β防御素可能是由于免疫状态改变所致。TNF-α、IL-6 和 IL-8 是在银屑病斑块中表达的常见细胞因子，以维持炎症循环。HBD-2、MMP-2 和 TNF-α 与银屑病的严重程度呈正相关。同时，IL-8 的表达随着临床上更严重的银屑病显著降低，这使得这些因素成为银屑病炎症的候选预后因素。
