Kusunoki H, Sakaue M, Kato S, Kanda S
Graduate School of Science and Technology, Kobe University, Japan.
J Exp Zool. 1989 Mar;249(3):322-8. doi: 10.1002/jez.1402490311.
The aim of the present study was to examine the effects of the elimination of energy substrate from the medium and the effects of the preincubation vessel, temperature, and time on the induction of the acrosome reaction in ejaculated goat spermatozoa in chemically defined medium. Washed spermatozoa were resuspended in Brackett-Oliphant (BO) medium or substrate-free BO medium to give a high concentration and preincubated in open test tubes or sealed glass tubes at 37.0 or 39.5 degrees C for 1, 2, or 3 h. Sperm acrosome reaction was evaluated using a simplified triple-stain technique and a hamster test. It was found that the goat sperm acrosome reaction occurred more readily in the sealed glass tubes than in open test tubes, in substrate-free BO medium than in BO medium, and at 39.5 than at 37.0 degrees C. During preincubation with substrate-free BO medium in sealed glass tubes at 39.5 degrees C, the optimum time necessary to induce the acrosome reaction in goat spermatozoa was 2-3 h.
本研究的目的是检测从培养基中去除能量底物的影响，以及预孵育容器、温度和时间对化学限定培养基中射精山羊精子顶体反应诱导的影响。将洗涤后的精子重悬于布拉克特-奥利芬特（BO）培养基或无底物BO培养基中，使其达到高浓度，并在开放试管或密封玻璃管中于37.0或39.5℃下预孵育1、2或3小时。使用简化的三重染色技术和仓鼠试验评估精子顶体反应。结果发现，山羊精子顶体反应在密封玻璃管中比在开放试管中更容易发生，在无底物BO培养基中比在BO培养基中更容易发生，在39.5℃时比在37.0℃时更容易发生。在39.5℃下于密封玻璃管中用无底物BO培养基预孵育期间，诱导山羊精子顶体反应所需的最佳时间为2 - 3小时。
