Bielfeld P, Jeyendran R S, Holmgren W J, Zaneveld L J
Department of Obstetrics and Gynecology, Rush University, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612.
J Androl. 1990 May-Jun;11(3):260-9.
Preincubation of human spermatozoa in an egg yolk medium (TESTY) at 5 C, followed by washing at 37 C by centrifugation and resuspension in a standard medium (BWW), enhanced the percentage of spermatozoa that underwent the acrosome reaction and increased sperm penetration into zona-free hamster oocytes, as compared with BWW treatment only. The difference in the occurrence of the acrosome reaction between the two treatment protocols was present whether the spermatozoa were incubated for 3 or for 18 h. The increase in acrosome reaction occurred only when spermatozoa were washed after TESTY treatment. Washing at 5 C was not as effective as washing at 37 C. No increased loss of acrosomes was observed when BWW-treated spermatozoa were subjected to the washing procedure. Ionophore A23187 stimulated the acrosome reaction of BWW-treated but not of TESTY-treated spermatozoa, whether or not they were washed before ionophore treatment. In the absence of egg yolk, the medium (TEST) caused only a small enhancement in the acrosome reaction as compared with BWW, but an increase occurred upon addition of ionophore A23187. We conclude that treatment with TESTY enhances the capacitation/acrosome reaction of human spermatozoa and that the removal of egg yolk after incubation, as well as the temperature shock, contribute to this effect.
将人类精子在5℃的蛋黄培养基（TESTY）中预孵育，随后在37℃下通过离心洗涤并重悬于标准培养基（BWW）中，与仅用BWW处理相比，经历顶体反应的精子百分比增加，并且精子穿透去透明带仓鼠卵母细胞的能力增强。无论精子孵育3小时还是18小时，两种处理方案在顶体反应发生率上的差异均存在。顶体反应的增加仅在TESTY处理后洗涤精子时出现。在5℃下洗涤不如在37℃下洗涤有效。当对BWW处理的精子进行洗涤程序时，未观察到顶体损失增加。离子载体A23187刺激BWW处理的精子而非TESTY处理的精子发生顶体反应，无论它们在离子载体处理前是否洗涤。在没有蛋黄的情况下，与BWW相比，培养基（TEST）仅使顶体反应略有增强，但添加离子载体A23187后会出现增加。我们得出结论，TESTY处理可增强人类精子的获能/顶体反应，并且孵育后去除蛋黄以及温度冲击有助于产生这种效果。
