Winner C O
Department of Zoology, Miami University, Oxford, Ohio 45056.
J Exp Zool. 1989 Mar;249(3):343-6. doi: 10.1002/jez.1402490315.
This paper describes the response of early four-digit regenerates of axolotls to reamputation and denervation. Reamputation of innervated regenerates led to sharp increases in 3H-thymidine labeling index (LI) and mitotic index (MI) on days 2-5 post-reamputation. This resembles the response of innervated limbs following initial amputation. Regenerates that were denervated at the time of reamputation exhibited no proliferative response through day 5. This is in marked contrast to denervated, original amputation limb stumps, in which LI and MI rise for several days (as in innervated stumps) before falling to background levels. Although myelin was scarce near the level of reamputation, the lack of proliferation cannot be explained solely on that basis. The results are consistent with the possibility that the "neurotrophic factor" that causes stump and blastema cell mitosis is not present in unamputated limbs but is made in response to amputation.
本文描述了蝾螈早期四指再生体对再次截肢和去神经支配的反应。对有神经支配的再生体进行再次截肢后，在再次截肢后的第2 - 5天，3H-胸腺嘧啶核苷标记指数（LI）和有丝分裂指数（MI）急剧增加。这类似于初次截肢后有神经支配的肢体的反应。在再次截肢时去神经支配的再生体在第5天之前没有表现出增殖反应。这与去神经支配的原始截肢肢体残端形成鲜明对比，在原始截肢肢体残端中，LI和MI会上升几天（如同有神经支配的残端），然后降至背景水平。尽管在再次截肢水平附近髓磷脂稀少，但增殖的缺乏不能仅基于此来解释。这些结果与以下可能性一致，即导致残端和芽基细胞有丝分裂的“神经营养因子”在未截肢的肢体中不存在，而是在截肢后产生的。
