Ganesan G, Warren R V, Leproux A, Compton M, Cutler K, Wittkopp S, Tran G, O'Sullivan T, Malik S, Galassetti P R, Tromberg B J
UC Irvine Beckman Laser Institute and Medical Clinic, Irvine, CA, USA.
UC Irvine Institute for Clinical and Translational Science, Irvine, CA, USA.
Int J Obes (Lond). 2016 Aug;40(8):1292-300. doi: 10.1038/ijo.2016.43. Epub 2016 Apr 22.
Changes in subcutaneous adipose tissue (AT) structure and metabolism have been shown to correlate with the development of obesity and related metabolic disorders. Measurements of AT physiology could provide new insight into metabolic disease progression and response to therapy. An emerging functional imaging technology, diffuse optical spectroscopic imaging (DOSI), was used to obtain quantitative measures of near infrared (NIR) AT optical and physiological properties.
Ten overweight or obese adults were assessed during 3 months on calorie-restricted diets. DOSI-derived tissue concentrations of hemoglobin, water and lipid and the wavelength-dependent scattering amplitude (A) and slope (b) obtained from 30 abdominal locations and three time points (T0, T6, T12) were calculated and analyzed using linear mixed-effects models and were also used to form 3D surface images.
Subjects lost a mean of 11.7±3.4% of starting weight, while significant changes in A (+0.23±0.04 mm(-1), adj. P<0.001),b (-0.17±0.04, adj. P<0.001), tissue water fraction (+7.2±1.1%, adj. P<0.001) and deoxyhemoglobin (1.1±0.3 μM, adj. P<0.001) were observed using mixed-effect model analysis.
Optical scattering signals reveal alterations in tissue structure that possibly correlate with reductions in adipose cell volume, while water and hemoglobin dynamics suggest improved AT perfusion and oxygen extraction. These results suggest that DOSI measurements of NIR optical and physiological properties could be used to enhance understanding of the role of AT in metabolic disorders and provide new strategies for diagnostic monitoring of obesity and weight loss.
皮下脂肪组织（AT）结构和代谢的变化已被证明与肥胖及相关代谢紊乱的发展相关。对AT生理学的测量可为代谢疾病的进展和治疗反应提供新的见解。一种新兴的功能成像技术，即漫射光谱成像（DOSI），被用于获取近红外（NIR）AT光学和生理特性的定量测量值。
对10名超重或肥胖成年人进行为期3个月的热量限制饮食评估。计算并使用线性混合效应模型分析从30个腹部位置和三个时间点（T0、T6、T12）获得的DOSI衍生的血红蛋白、水和脂质的组织浓度以及波长依赖性散射幅度（A）和斜率（b），并用于形成三维表面图像。
受试者平均体重减轻了初始体重的11.7±3.4%，同时使用混合效应模型分析观察到A（+0.23±0.04 mm(-1)，校正P<0.001）、b（-0.17±0.04，校正P<0.001）、组织水分分数（+7.2±1.1%，校正P<0.001）和脱氧血红蛋白（1.1±0.3 μM，校正P<0.001）有显著变化。
光学散射信号揭示了组织结构的改变，这可能与脂肪细胞体积的减小相关，而水和血红蛋白的动态变化表明AT灌注和氧摄取得到改善。这些结果表明，对NIR光学和生理特性的DOSI测量可用于增强对AT在代谢紊乱中的作用的理解，并为肥胖和体重减轻的诊断监测提供新策略。
