He Qiyang, Xia Qianjun, Wang Yuejiao, Li Xun, Zhang Yu, Hu Bo, Wang Fei
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, P.R. China.
College of Chemical Engineering, Jiangsu Key Laboratory of Biomass-Based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing 210037, P.R. China.
J Microbiol Biotechnol. 2016 Jul 28;26(7):1278-84. doi: 10.4014/jmb.1601.01075.
Rhizopus chinensis cells immobilized on loofah (Luffa cylindrica) sponges were used to produce biodiesel via the transesterification of soybean oil. In whole-cell immobilization, loofah sponge is considered to be a superior alternative to conventional biomass carriers because of its biodegradable and renewable properties. During cell cultivation, Rhizopus chinensis mycelia can spontaneously and firmly adhere to the surface of loofah sponge particles. The optimal conditions for processing 9.65 g soybean oil at 40°C and 180 rpm using a 3:1 methanol-to-oil molar ratio were found to be 8% cell addition and 3-10% water content (depending on the oil's weight). Under optimal conditions, an over 90% methyl ester yield was achieved after the first reaction batch. The operational stability of immobilized Rhizopus chinensis cells was assayed utilizing a 1:1 methanol-to-oil molar ratio, thus resulting in a 16.5-fold increase in half-life when compared with immobilized cells of the widely studied Rhizopus oryzae. These results suggest that transesterification of vegetable oil using Rhizopus chinensis whole cells immobilized onto loofah sponge is an effective approach for biodiesel production.
固定在丝瓜（Luffa cylindrica）海绵上的华根霉细胞被用于通过大豆油的酯交换反应生产生物柴油。在全细胞固定化过程中，丝瓜海绵因其可生物降解和可再生的特性，被认为是传统生物质载体的优质替代品。在细胞培养过程中，华根霉菌丝体能自发且牢固地附着在丝瓜海绵颗粒表面。使用3:1的甲醇与油摩尔比，在40°C和180 rpm条件下处理9.65 g大豆油的最佳条件为添加8%的细胞和3 - 10%的含水量（取决于油的重量）。在最佳条件下，第一批反应后甲酯产率超过90%。使用1:1的甲醇与油摩尔比测定了固定化华根霉细胞的操作稳定性，结果表明，与广泛研究的米根霉固定化细胞相比，其半衰期增加了16.5倍。这些结果表明，使用固定在丝瓜海绵上的华根霉全细胞进行植物油的酯交换反应是生产生物柴油的有效方法。
