Oetama Vincensius S P, Hennersdorf Philipp, Abdul-Aziz Muslihudeen A, Mrotzek Grit, Haryanti Haryanti, Saluz Hans Peter
Leibniz-Institute for Natural Product Research and Infection Biology, Beutenbergstr. 11a, D-07745 Jena, Germany; Friedrich Schiller University of Jena, Fürstengraben 1, D-07743 Jena, Germany.
Friedrich Schiller University of Jena, Fürstengraben 1, D-07743 Jena, Germany; Australian Centre for Ancient DNA, University of Adelaide, Adelaide, South Australia, Australia.
Mar Pollut Bull. 2016 Sep 30;110(2):718-25. doi: 10.1016/j.marpolbul.2016.03.043. Epub 2016 Apr 15.
Penaeus monodon, the Asian black tiger shrimp is one of the most widely consumed marine crustaceans worldwide. In this study, we examine and compare the fecal microbiota of P. monodon from highly polluted waters around Jakarta Bay, with those of less polluted waters of Bali. Using next generation sequencing techniques, we identified potential bacterial pathogens and common viral diseases of shrimp. Proteobacteria (96.08%) was found to be the most predominant phylum, followed by Bacteriodetes (2.32%), Fusobacteria (0.96%), and Firmicutes (0.53%). On the order level, Vibrionales (66.20%) and Pseudoaltermonadales (24.81%) were detected as predominant taxa. qPCR profiling was used as a confirmatory step and further revealed Vibrio alginolyticus and Photobacterium damselae as two potential pathogenic species present in most of the samples. In addition, viral diseases for shrimp were discovered among the samples, WSSV in Jakarta free-living samples, YHV in Bali free-living samples and IHHNV in both.
斑节对虾，即亚洲黑虎虾，是全球消费最为广泛的海洋甲壳类动物之一。在本研究中，我们对来自雅加达湾周边高污染水域的斑节对虾与巴厘岛污染程度较低水域的斑节对虾的粪便微生物群进行了检测和比较。利用新一代测序技术，我们鉴定了虾类潜在的细菌病原体和常见病毒病。发现变形菌门（96.08%）是最主要的门类，其次是拟杆菌门（2.32%）、梭杆菌门（0.96%）和厚壁菌门（0.53%）。在目水平上，弧菌目（66.20%）和假交替单胞菌目（24.81%）被检测为主要分类群。定量聚合酶链反应分析用作验证步骤，并进一步揭示溶藻弧菌和美人鱼发光杆菌是大多数样本中存在的两种潜在致病物种。此外，在样本中发现了虾类的病毒病，雅加达自由生活样本中的白斑综合征病毒、巴厘岛自由生活样本中的黄头病毒以及两者中均有的传染性皮下和造血组织坏死病毒。
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