Wang Ruixuan, Guo Zihan, Tang Yapeng, Kuang Jiawei, Duan Yafei, Lin Heizhao, Jiang Shigui, Shu Hu, Huang Jianhua
Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen, 518121, China.
School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, 521041, China.
AMB Express. 2020 Jun 5;10(1):109. doi: 10.1186/s13568-020-01041-3.
Shrimp production is the second ranked of the most-traded production in these decades and the whiteleg shrimp Litopenaeus vannamei is the sixth most cultured species. Probiotics are alternative strategy for the promotion of growth and prevention of diseases in aquaculture. To confirm the effects of the probiotics on development and microbial community of L. vannamei larvae during different development stages, five kinds of probiotics (10 ~ 10 CFU/g) were added into the rearing environment of shrimp larvae, and the effects of probiotics on bacterial community and water quality, larval growth and immune index were determined from nauplius larval stage to post larval stage. Results suggested that probiotics treated groups showed larger survival rate than the control groups from Z1 stage to P5 stage. Lactobacillus could improve the larvae's survival ability, especially in the larval stages M2, M3, P1, P5 stage. It was confirmed that probiotics could promote the growth and development of shrimp larvae and prevent the incomplete molting in their growing process, particularly for EM-treated group. Results suggested that all the probiotics-treated groups had shown significant decreasing trend in the quantity of vibrios, except for the SA-treated group. And different probiotics could inhibit vibrios during different life periods. Among these probiotics, LA, EM and PB had shown the best effects, including improving survival rate of the larvae, promoting the larval metamorphosis, reducing the quantity of vibrios and NH-N and NO-N levels, and increasing bacterial diversity.
对虾养殖产量在过去几十年中是第二大贸易量的养殖产品，凡纳滨对虾是第六大养殖品种。益生菌是水产养殖中促进生长和预防疾病的替代策略。为了证实益生菌在凡纳滨对虾幼体不同发育阶段对其发育和微生物群落的影响，将五种益生菌（10⁶~10⁸CFU/g）添加到对虾幼体的养殖环境中，测定了从无节幼体期到仔虾期益生菌对细菌群落、水质、幼体生长和免疫指标的影响。结果表明，从Z1期到P5期，益生菌处理组的存活率高于对照组。乳酸杆菌能提高幼体的生存能力，尤其是在M2、M3、P1、P5幼体阶段。证实益生菌可促进对虾幼体的生长发育，防止其生长过程中的不完全蜕皮，特别是EM处理组。结果表明，除SA处理组外，所有益生菌处理组的弧菌数量均呈显著下降趋势。不同的益生菌在不同的生命时期对弧菌有抑制作用。在这些益生菌中，LA、EM和PB表现出最好的效果，包括提高幼体存活率、促进幼体变态、减少弧菌数量以及NH₃-N和NO₂-N水平，并增加细菌多样性。
