Harrison Robert L, Rowley Daniel L, Keena Melody A
Invasive Insect Biocontrol and Behavior Laboratory, Beltsville Agricultural Research Center, USDA Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.
Northern Research Station, USDA Forest Service, 51 Mill Pond Road, Hamden, CT 06514, USA.
J Invertebr Pathol. 2016 Jun;137:10-22. doi: 10.1016/j.jip.2016.03.014. Epub 2016 Apr 28.
Isolates of the baculovirus species Lymantria dispar multiple nucleopolyhedrovirus have been formulated and applied to suppress outbreaks of the gypsy moth, L. dispar. To evaluate the genetic diversity in this species at the genomic level, the genomes of three isolates from Massachusetts, USA (LdMNPV-Ab-a624), Spain (LdMNPV-3054), and Japan (LdMNPV-3041) were sequenced and compared with four previously determined LdMNPV genome sequences. The LdMNPV genome sequences were collinear and contained the same homologous repeats (hrs) and clusters of baculovirus repeat orf (bro) gene family members in the same relative positions in their genomes, although sequence identities in these regions were low. Of 146 non-bro ORFs annotated in the genome of the representative isolate LdMNPV 5-6, 135 ORFs were found in every other LdMNPV genome, including the 37 core genes of Baculoviridae and other genes conserved in genus Alphabaculovirus. Phylogenetic inference with an alignment of the core gene nucleotide sequences grouped isolates 3041 (Japan) and 2161 (Korea) separately from a cluster containing isolates from Europe, North America, and Russia. To examine phenotypic diversity, bioassays were carried out with a selection of isolates against neonate larvae from three European gypsy moth (Lymantria dispar dispar) and three Asian gypsy moth (Lymantria dispar asiatica and Lymantria dispar japonica) colonies. LdMNPV isolates 2161 (Korea), 3029 (Russia), and 3041 (Japan) exhibited a greater degree of pathogenicity against all L. dispar strains than LdMNPV from a sample of Gypchek. This study provides additional information on the genetic diversity of LdMNPV isolates and their activity against the Asian gypsy moth, a potential invasive pest of North American trees and forests.
舞毒蛾多核型多角体病毒（Lymantria dispar multiple nucleopolyhedrovirus）的分离株已被制备并应用于抑制舞毒蛾（L. dispar）的爆发。为了在基因组水平评估该物种的遗传多样性，对来自美国马萨诸塞州（LdMNPV-Ab-a624）、西班牙（LdMNPV-3054）和日本（LdMNPV-3041）的三个分离株的基因组进行了测序，并与之前测定的四个舞毒蛾多核型多角体病毒基因组序列进行了比较。舞毒蛾多核型多角体病毒的基因组序列是共线的，并且在其基因组的相同相对位置包含相同的同源重复序列（hrs）和杆状病毒重复开放阅读框（bro）基因家族成员簇，尽管这些区域的序列同一性较低。在代表性分离株LdMNPV 5-6的基因组中注释的146个非bro开放阅读框中，在其他每个舞毒蛾多核型多角体病毒基因组中都发现了135个开放阅读框，包括杆状病毒科的37个核心基因和在甲型杆状病毒属中保守的其他基因。通过对核心基因核苷酸序列进行比对的系统发育推断，将分离株3041（日本）和2161（韩国）与包含来自欧洲、北美和俄罗斯的分离株的一个簇分开。为了检测表型多样性，对一系列分离株针对来自三个欧洲舞毒蛾（Lymantria dispar dispar）和三个亚洲舞毒蛾（Lymantria dispar asiatica和Lymantria dispar japonica）群体的初孵幼虫进行了生物测定。与来自Gypchek样本的舞毒蛾多核型多角体病毒相比，分离株2161（韩国）、3029（俄罗斯）和304（日本）对所有舞毒蛾菌株表现出更高程度的致病性。本研究提供了关于舞毒蛾多核型多角体病毒分离株的遗传多样性及其对亚洲舞毒蛾（北美树木和森林的一种潜在入侵害虫）活性的更多信息。
