Connell Sean, Li Jianming, Durkes Abigail, Zaroura Mohammed, Shi Riyi
Medtric Biotech, LLC, West Lafayette, Indiana.
Center for Paralysis Research, Purdue University, West Lafayette, Indiana, 47907.
Wound Repair Regen. 2016 Jul;24(4):669-78. doi: 10.1111/wrr.12436. Epub 2016 Jun 3.
Increased microbial burden within the wound often complicates wound healing and may lead to subsequent infection or delayed healing. Here, we investigate a novel topical for addressing wound contamination that utilizes hyperosmotic saccharides with a cell membrane disrupting emulsion. These hyperosmotic nanoemulsions (HNE) were administered topically in a full-thickness biopsy model of wound healing. Results show that HNE were well tolerated in noninfected animals with no indications of dermal irritation or acute toxicity. Additionally, HNE was able to reduce bacterial bioburden (Escherichia coli and Enterococcus faecalis) levels by 3 logs within 24 h when wounds were inoculated with 5 × 10(6) total CFU. These bactericidal values were similar to wounds treated with silver sulfadiazine. Wound closure showed HNE wounds closed in 7.6 ± 0.2 days while SSD and control required 10.2 ± 0.4 and 10.4 ± 0.3 days, respectively. HNE maintained a moist wound environment, were well debrided, and exhibited improved hemostatic response. Further histological examination revealed enhanced granulation tissue as compared to silver sulfadiazine and control cohorts. These results were corroborated with 3D topographical imprints of the wounds at day 14 which qualitatively showed a smoother surface. In contrast, silver sulfadiazine appeared to delay wound closure. Finally, dermal sensitization and irritation studies conducted in guinea pig and rabbits did not reveal any acute dermal side effects from HNE exposure. The cumulative data indicates nonantibiotic-based HNEs may be a promising topical treatment for the management of contaminated wounds.
伤口内微生物负荷增加常常使伤口愈合复杂化，并可能导致后续感染或愈合延迟。在此，我们研究了一种用于解决伤口污染的新型局部用药，它利用具有细胞膜破坏作用的乳液形式的高渗糖类。这些高渗纳米乳液（HNE）在全层活检伤口愈合模型中进行局部给药。结果表明，HNE在未感染动物中耐受性良好，没有皮肤刺激或急性毒性的迹象。此外，当伤口接种5×10⁶总CFU时，HNE能够在24小时内将细菌生物负荷（大肠杆菌和粪肠球菌）水平降低3个对数。这些杀菌值与用磺胺嘧啶银处理的伤口相似。伤口闭合情况显示，HNE处理的伤口在7.6±0.2天愈合，而磺胺嘧啶银组和对照组分别需要10.2±0.4天和10.4±0.3天。HNE保持伤口湿润环境，清创良好，并表现出改善的止血反应。进一步的组织学检查显示，与磺胺嘧啶银组和对照组相比，肉芽组织增强。这些结果在第14天伤口的3D地形印记中得到证实，定性显示表面更光滑。相比之下，磺胺嘧啶银似乎延迟了伤口闭合。最后，在豚鼠和兔子身上进行的皮肤致敏和刺激研究未发现HNE暴露有任何急性皮肤副作用。累积数据表明，基于非抗生素的HNE可能是一种有前途的用于处理污染伤口的局部治疗方法。
