Connell Sean, Li Jianming, Durkes Abigail, Freeman Lynetta
Medtric Biotech, LLC, West Lafayette, Indiana.
Department of Basic Medical Sciences, Center for Paralysis Research, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana.
Adv Wound Care (New Rochelle). 2017 May 1;6(5):153-165. doi: 10.1089/wound.2016.0689.
In this work, we introduce a novel hyperosmotic nanoemulsion (HNE) topical agent for use in wound healing. These topical emulsion complexes combine a lipophilic thymol nanoemulsion with a hyperosmotic saccharide matrix. This combination has been previously shown to possess synergistic antimicrobial activity against a host of common and drug-resistant pathogens . In this study, we present additional data to assess the safety and efficacy of these emulsions in a partial thickness injury model in swine. Ten wounds sized 2 × 3.5 cm were created in 18 pigs using an electrodermatome set at a depth of 0.76 mm. The wounds were subsequently contaminated with a cocktail of , , , and at 5 × 10 total colony forming unit per wound. Treatments were subdivided in the control group and emulsion concentrations at 0.0%, 0.01%, 0.03%, and 0.063% thymol content. Longitudinal metrics for wound healing included rate of reepithelialization, wound bed color measurements, amount of wound exudate, wound swab culture data, and histological examination at 4, 7, and 14 days. The cosmetics of the healed wound were obtained at day 14 with three-dimensional photogrammetry. Experimental results showed that HNE reduced the wound level bacteria count by ∼0.5-1 log versus controls after 24 h. The amount of pathogen reduction was weakly correlated to the concentration of the emulsion. In addition, all HNE groups maintained a moist wound environment and showed increased fibrin formation and improved hemostatic response. No significant difference in the rate of reepithelialization or wound closure was found between treatment concentrations and control groups. HNE treatment did not demonstrate any adverse host tissue response. These results suggest HNE may be a candidate for reducing wound bacterial counts without compromising reepithelialization.
在本研究中，我们介绍了一种用于伤口愈合的新型高渗纳米乳剂（HNE）局部用药。这些局部乳剂复合物将亲脂性百里酚纳米乳剂与高渗糖基质结合在一起。先前已证明这种组合对多种常见和耐药病原体具有协同抗菌活性。在本研究中，我们提供了更多数据，以评估这些乳剂在猪的部分厚度损伤模型中的安全性和有效性。使用设置为0.76毫米深度的电动皮肤刀在18头猪身上制造了10个大小为2×3.5厘米的伤口。随后，每个伤口用含有、、、和的混合物以每伤口5×10个总菌落形成单位进行污染。治疗分为对照组和百里酚含量为0.0%、0.01%、0.03%和0.063%的乳剂浓度组。伤口愈合的纵向指标包括上皮再形成率、伤口床颜色测量、伤口渗出物量、伤口拭子培养数据以及在第4、7和14天的组织学检查。在第14天通过三维摄影测量获得愈合伤口的外观。实验结果表明，与对照组相比，HNE在24小时后使伤口细菌数量减少了约0.5-1个对数。病原体减少量与乳剂浓度呈弱相关。此外，所有HNE组均保持伤口湿润环境，并显示纤维蛋白形成增加和止血反应改善。治疗浓度组与对照组在上皮再形成率或伤口闭合率方面未发现显著差异。HNE治疗未显示任何不良宿主组织反应。这些结果表明，HNE可能是一种在不影响上皮再形成的情况下减少伤口细菌数量的候选药物。
