Kubin Minna E, Hägg Päivi M, Kokkonen Nina, Haapasaari Kirsi-Maria, Väyrynen Juha P, Uchida Tatsuya, Glumoff Virpi, Kulmala Petri, Hurskainen Tiina, Tasanen Kaisa
Department of Dermatology, University of Oulu, FIN-90029 Oulu, Finland.
Acta Derm Venereol. 2016 Nov 2;96(7):922-926. doi: 10.2340/00015555-2443.
First-line treatments of bullous pemphigoid (BP) are topical and systemic glucocorticoids (GC). The actions of GC are mediated by glucocorticoid receptors (GR), which exist in several isoforms, of which GRα and GRβ are the most important. In many inflammatory diseases, up-regulation of GRβ is associated with GC insensitivity. The aims of this study were to determine the expression of GRα and GRβ in patients with BP and to investigate the effect of prednisolone treatment on the expression of GR isoforms in BP. Quantitative real-time PCR (qPCR) analysis demonstrated that GR isoform mRNAs are expressed in peripheral blood mononuclear cells (PBMC) from patients with BP. Expression of GRα and GRβ protein was confirmed by immunohistochemical staining of BP skin biopsies and by Western blot analysis and flow cytometric analysis of PBMCs. During prednisolone treatment, GRα and GRβ expression varied markedly, but changes were not suitable as a clinical marker of GC sensitivity in patients with BP.
大疱性类天疱疮（BP）的一线治疗方法是局部和全身使用糖皮质激素（GC）。GC的作用是由糖皮质激素受体（GR）介导的，GR存在几种亚型，其中GRα和GRβ最为重要。在许多炎症性疾病中，GRβ的上调与GC不敏感有关。本研究的目的是确定BP患者中GRα和GRβ的表达，并研究泼尼松龙治疗对BP患者GR亚型表达的影响。定量实时PCR（qPCR）分析表明，GR亚型mRNA在BP患者的外周血单个核细胞（PBMC）中表达。通过BP皮肤活检的免疫组织化学染色、PBMC的蛋白质免疫印迹分析和流式细胞术分析证实了GRα和GRβ蛋白的表达。在泼尼松龙治疗期间，GRα和GRβ的表达有明显变化，但这些变化不适宜作为BP患者GC敏感性的临床标志物。
