Department of Materials Science & Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, Germany.
Department of Gynaecology & Obstetrics, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University-Clinic Erlangen, Universitätsstraße 21-23, 91054 Erlangen, Germany.
Nanomedicine (Lond). 2016 May;11(9):1093-105. doi: 10.2217/nnm.16.20. Epub 2016 Apr 13.
There is limited knowledge regarding differentiation of osteoclasts in the presence of nanoscale bioactive glass (nBG). This investigation examined increasing concentrations of 45S5 nBG and their influence on osteoclast differentiation.
MATERIALS & METHODS: Different concentrations of 45S5 nBG were cultured up to 14 days with the murine RAW264.7 cell line and human primary monocytes cultured with M-CSF and RANKL.
Culturing cells for 14 days with 500 μg/ml nBG showed a viability of 100%; however DNA synthesis was reduced, supporting differentiation into osteoclast-like cells. Using RAW cells, activation of nine genes, including cell fusion genes, occurred in an nBG concentration dependent manner. Low concentrations of nBG increased expression of genes involved in commitment to cell fusion, whereas high concentrations increased gene expression supporting osteoclast-like differentiation.
nBG enhances both RAW264.7 and human osteoclast differentiation. nBG controlled gene expression in a concentration dependent manner could reflect normal regulation during bone growth.
关于纳米生物活性玻璃（nBG）存在时破骨细胞的分化，目前的知识有限。本研究检测了不同浓度的 45S5 nBG 及其对破骨细胞分化的影响。
将不同浓度的 45S5 nBG 与鼠 RAW264.7 细胞系和用 M-CSF 和 RANKL 培养的人原代单核细胞共培养 14 天。
用 500μg/ml nBG 培养细胞 14 天，细胞活力为 100%；然而，DNA 合成减少，支持向破骨细胞样细胞分化。在 RAW 细胞中，九个基因的激活，包括细胞融合基因，以 nBG 浓度依赖的方式发生。低浓度的 nBG 增加了参与细胞融合的基因的表达，而高浓度的 nBG 增加了支持破骨细胞样分化的基因表达。
nBG 增强了 RAW264.7 和人破骨细胞的分化。nBG 以浓度依赖的方式控制基因表达，这可能反映了骨生长过程中的正常调节。
