C57BL/6J小鼠耳蜗中大电导钙激活钾通道表达的下调
Down-regulation of the large conductance Ca(2+)-activated K(+) channel expression in C57BL/6J cochlea.
作者信息
Pan Chunchen, Chu Hanqi, Lai Yanbing, Liu Yun, Sun Yanbo, Du Zhihui, Chen Jin, Tong Ting, Chen Qingguo, Zhou Liangqiang, Bing Dan, Tao Yanling
机构信息
a Department of Otolaryngology-Head and Neck Surgery , Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , PR China ;
b Department of Otolaryngology-Head and Neck Surgery , the First People's Hospital of Foshan , Foshan , Guangdong , PR China.
出版信息
Acta Otolaryngol. 2016 Sep;136(9):875-8. doi: 10.3109/00016489.2016.1168941. Epub 2016 Apr 19.
CONCLUSION
The large conductance Ca(2+)-activated K(+ )channels (BK) expression is decreased in the cochleae of age-related hearing loss (AHL) mice. BK channel may be associated with AHL.
OBJECTIVE
AHL is the most common among elderly persons. BK channels act as sensors for membrane voltage and intracellular Ca(2+ )and are essential for hearing. To investigate the distribution of BK channel in the cochleae of C57BL/6J mice, and the relationship between the expression of BK channel and the etiology of AHL.
METHODS
BK expression was studied in the cochleae of C57BL/6J mice at various ages (4, 12, 26, 52 weeks). The expressions of BK at the protein and mRNA levels were detected by immunofluorescence technique, western blot and quantitative real time PCR.
RESULTS
In comparison with 4-week-old mice, BK expressions in the cochleae at 12, 26 and 52 weeks of age were significantly and gradually decreased at both the protein and the mRNA levels. The immunofluorescence technique showed the BK channel was located in the hair cells and cells of the spiral ganglion, spiral ligament and stria vascularis and its expression also decreased with aging.
结论
在年龄相关性听力损失（AHL）小鼠的耳蜗中，大电导钙激活钾（BK）通道的表达降低。BK通道可能与AHL有关。
目的
AHL在老年人中最为常见。BK通道作为膜电压和细胞内钙的传感器，对听力至关重要。研究BK通道在C57BL/6J小鼠耳蜗中的分布，以及BK通道表达与AHL病因之间的关系。
方法
研究不同年龄（4、12、26、52周）的C57BL/6J小鼠耳蜗中的BK表达。采用免疫荧光技术、蛋白质印迹法和定量实时PCR检测BK在蛋白质和mRNA水平的表达。
结果
与4周龄小鼠相比，12、26和52周龄小鼠耳蜗中BK的蛋白质和mRNA水平均显著且逐渐降低。免疫荧光技术显示BK通道位于毛细胞、螺旋神经节、螺旋韧带和血管纹的细胞中，其表达也随年龄增长而降低。
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