酶联免疫吸附测定法检测白纹伊蚊和三带喙库蚊（双翅目：蚊科）体内犬恶丝虫（旋尾目：丝虫科）幼虫的抗原。

Enzyme-linked immunosorbent assay to detect antigens of Dirofilaria immitis (Spirunida: Filariidae) larvae in Aedes albopictus and Culex tritaeniorhynchus (Diptera: Culicidae).

作者信息

Konishi E

出版信息

J Med Entomol. 1989 Mar;26(2):113-7. doi: 10.1093/jmedent/26.2.113.

DOI:10.1093/jmedent/26.2.113

Abstract

An enzyme-linked immunosorbent assay (ELISA) method was developed for detecting the larval antigens of Dirofilaria immitis (Leidy) in Aedes albopictus (Skuse) and Culex tritaeniorhynchus Giles to replace the conventional dissection method. The assay was sensitive enough to detect 21.3 ng/ml (2.13 ng per microplate well) or more of the soluble antigen obtained from adult worms and an antigen amount corresponding to at least one larva at any developing stage. No inhibitory effect of host tissues was observed on the ELISA reaction even when the homogenate of 20 mosquitoes was included in the 1-ml ELISA diluent. Moreover, third-stage larval antigen could be detected readily when mixed with up to 100 mosquito heads. The ELISA method has been effective in field surveys of mosquito populations with low filarial infection rates.

摘要

开发了一种酶联免疫吸附测定（ELISA）方法，用于检测白纹伊蚊（Skuse）和三带喙库蚊（Giles）体内犬恶丝虫（Leidy）的幼虫抗原，以取代传统的解剖方法。该测定法灵敏度足够高，能够检测到从成虫获得的21.3 ng/ml（每微孔板孔2.13 ng）或更多的可溶性抗原，以及对应于任何发育阶段至少一条幼虫的抗原量。即使在1 ml ELISA稀释液中加入20只蚊子的匀浆，也未观察到宿主组织对ELISA反应有抑制作用。此外，当与多达100个蚊头混合时，很容易检测到第三期幼虫抗原。ELISA方法在丝虫感染率低的蚊虫种群现场调查中很有效。