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[微小RNA-191在T淋巴母细胞白血病/淋巴瘤中的表达及其潜在机制]

[Expression of microRNA-191 in T lymphoblastic leukemia/lymphoma and its underlying mechanism].

作者信息

Zhang Jinghang, Yang Xaioyu, Li Min, Huang Xin, Liu Cuiling, Gao Zifen

机构信息

Department of Pathology, Peking University, HSC, Beijing 100191, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2016 Apr;37(4):273-7. doi: 10.3760/cma.j.issn.0253-2727.2016.04.003.

DOI:10.3760/cma.j.issn.0253-2727.2016.04.003
PMID:27093985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7343091/
Abstract

OBJECTIVE

To evaluate the correlation between MicroRNA-191 (miR-191) and T lymphoblastic leukemia/lymphoma (T-ALL/LBL) to probe its underlying molecular mechanism.

METHODS

The expression of miR-191 was examined by real-time PCR (RT-PCR) in 20 T-ALL/LBL tissue samples and 20 lymphoid reactive hyperplasia (LRH) tissue samples. The correlation between miR-191 and the clinicopathological feature of T-ALL/LBL was analyzed. Antisense miR-191 lentiviral vectors was constructed and transfected into T-ALL/LBL Jukat cells. After transfection, the expression of miR-191 was examined by RT-PCR. The cell activity was evaluated by CCK-8 asssy. The cell cycle and apoptosis were determined by flow cytometry.

RESULTS

Compared with LRH samples, the results of RT-PCR showed significant upregulation of miR-191 in 20 T-ALL/LBL tissue samples (1.875±0.079 vs 1.000, P<0.05). The expression level of miR-191 was negatively associated with prognosis. Compared with LV-NC-GFP and control groups, the expression of miR-191 significantly decreased after transfection of antisense miR-191 lentiviral vectors (0.578±0.012 vs 1.011±0.053 and 1.000, P<0.05), the percentages of apoptotic cells and the cell in G0/G1 phase significantly increased (P<0.05).

CONCLUSIONS

miR-191 might play a significant role in the development of T-ALL/LBL, implicating a new target for therapy.

摘要

目的

评估微小RNA-191（miR-191）与T淋巴母细胞白血病/淋巴瘤（T-ALL/LBL）之间的相关性，以探究其潜在分子机制。

方法

采用实时定量聚合酶链反应（RT-PCR）检测20例T-ALL/LBL组织样本和20例淋巴反应性增生（LRH）组织样本中miR-191的表达。分析miR-191与T-ALL/LBL临床病理特征的相关性。构建反义miR-191慢病毒载体并转染至T-ALL/LBL Jukat细胞。转染后，通过RT-PCR检测miR-191的表达。采用CCK-8法评估细胞活性。通过流式细胞术检测细胞周期和凋亡情况。

结果

与LRH样本相比，RT-PCR结果显示20例T-ALL/LBL组织样本中miR-191显著上调（1.875±0.079对1.000，P<0.05）。miR-191的表达水平与预后呈负相关。与LV-NC-GFP组和对照组相比，转染反义miR-191慢病毒载体后miR-191的表达显著降低（0.578±0.012对1.011±0.053和1.000，P<0.05），凋亡细胞百分比和G0/G1期细胞显著增加（P<0.05）。

结论

miR-191可能在T-ALL/LBL的发生发展中起重要作用，提示其可能成为新的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/507d/7343091/35484753bd66/cjh-37-04-273-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/507d/7343091/bb3ae813acdb/cjh-37-04-273-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/507d/7343091/92cc070b7050/cjh-37-04-273-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/507d/7343091/35484753bd66/cjh-37-04-273-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/507d/7343091/bb3ae813acdb/cjh-37-04-273-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/507d/7343091/92cc070b7050/cjh-37-04-273-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/507d/7343091/35484753bd66/cjh-37-04-273-g003.jpg

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