Dickson G R, McKenna S, McColl K, Carr K E
Department of Anatomy, Queen's University of Belfast, U.K.
J Microsc. 1989 Jan;153(Pt 1):75-9. doi: 10.1111/j.1365-2818.1989.tb01468.x.
A rapid method is described whereby gastrointestinal biopsy specimen surfaces can be examined by scanning electron microscopy with overlying tract contents (debris) intact, and also re-examined after cleaning to determine the structure of the underlying mucosal surface. The conductive coating of gold is removed using mercury, a non-wetting agent. The specimen surface is suitably cleaned of debris after a brief ultrasonication in absolute ethanol which mixes with the transitional fluid (CO2) used for critical point drying.
本文描述了一种快速方法，通过该方法可以在胃肠道活检标本表面覆盖的消化道内容物（碎片）保持完整的情况下，利用扫描电子显微镜对其进行检查，并且在清洁后还可再次检查以确定其下方黏膜表面的结构。使用非润湿性试剂汞去除金导电涂层。在与用于临界点干燥的过渡流体（二氧化碳）混合的无水乙醇中进行短暂超声处理后，适当清洁标本表面的碎片。
