Tzounakas Vassilis L, Kriebardis Anastasios G, Papassideri Issidora S, Antonelou Marianna H
Department of Cell Biology and Biophysics, Faculty of Biology, NKUA, Athens, Greece.
Department of Medical Laboratories, Faculty of Health and Caring Professions, Technological and Educational Institute of Athens, Greece.
Proteomics Clin Appl. 2016 Aug;10(8):791-804. doi: 10.1002/prca.201500128. Epub 2016 May 23.
Although the molecular pathways leading to the progressive deterioration of stored red blood cells (RBC storage lesion) and the clinical relevance of storage-induced changes remain uncertain, substantial donor-specific variability in RBC performance during storage, and posttransfusion has been established ("donor-variation effect"). In-bag hemolysis and numerous properties of the RBC units that may affect transfusion efficacy have proved to be strongly donor-specific. Donor-variation effect may lead to the production of highly unequal blood labile products even when similar storage strategy and duration are applied. Genetic, undiagnosed/subclinical medical conditions and lifestyle factors that affect RBC characteristics at baseline, including RBC lifespan, energy metabolism, and sensitivity to oxidative stress, are all likely to influence the storage capacity of individual donors' cells, although not evident by the donor's health or hematological status at blood donation. Consequently, baseline characteristics of the donors, such as membrane peroxiredoxin-2 and serum uric acid concentration, have been proposed as candidate biomarkers of storage quality. This review article focuses on specific factors that might contribute to the donor-variation effect and emphasizes the emerging need for using omics-based technologies in association with in vitro and in vivo transfusion models and clinical trials to discover biomarkers of storage quality and posttransfusion recovery in donor blood.
尽管导致储存红细胞逐渐恶化（红细胞储存损伤）的分子途径以及储存诱导变化的临床相关性仍不明确，但已证实红细胞在储存期间以及输血后的性能存在显著的供体特异性差异（“供体变异效应”）。袋内溶血以及可能影响输血疗效的红细胞单位的众多特性已被证明具有很强的供体特异性。即使采用相似的储存策略和持续时间，供体变异效应也可能导致产生高度不均一的血液不稳定产品。影响红细胞基线特征（包括红细胞寿命、能量代谢和对氧化应激的敏感性）的遗传因素、未诊断/亚临床疾病状况和生活方式因素，都可能影响个体供体细胞的储存能力，尽管在献血时供体的健康状况或血液学状态并不明显。因此，已提出供体的基线特征，如膜过氧化物酶2和血清尿酸浓度，作为储存质量的候选生物标志物。这篇综述文章聚焦于可能导致供体变异效应的特定因素，并强调迫切需要将基于组学的技术与体外和体内输血模型以及临床试验相结合，以发现供体血液中储存质量和输血后恢复的生物标志物。
