Tzounakas Vassilis L, Karadimas Dimitrios G, Anastasiadi Alkmini T, Georgatzakou Hara T, Kazepidou Eleftheria, Moschovas Dimitris, Velentzas Athanassios D, Kriebardis Anastasios G, Zafeiropoulos Nikolaos E, Avgeropoulos Apostolos, Lekka Marilena, Stamoulis Konstantinos E, Papassideri Issidora S, Antonelou Marianna H
Department of Biology, School of Science, National & Kapodistrian University of Athens (NKUA), Athens, Greece.
Department of Chemistry, School of Science, University of Ioannina, Ioannina, Greece.
Transfusion. 2018 Jan;58(1):34-40. doi: 10.1111/trf.14379. Epub 2017 Oct 23.
Previous investigations in leukoreduced units of red blood cells (RBCs) in mannitol additive solution revealed the close association of uric acid (UA) levels in vivo with the susceptibility of RBCs to storage lesion markers. In this study, we examined whether UA has a similar correlation with the capability of RBCs to cope with the oxidative provocations of storage under different conditions, namely, in CPDA-1 and in the absence of leukoreduction.
The UA-dependent antioxidant capacity of the supernatant was measured in nonleukoreduced units of RBCs in CPDA (n = 47). The possible effect of UA variability on the storage lesion profile was assessed by monitoring several physiologic properties of RBCs and supernatant, including cell shape, reactive oxygen species, and size distribution of extracellular vesicles, in units exhibiting the lowest or highest levels of UA activity (n = 16) among donors, throughout the storage period.
In stored RBC units, the UA-dependent antioxidant activity of the supernatant declined as a function of storage duration but always in strong relation to the UA levels in fresh blood. Contrary to units of poor-UA activity, RBCs with the highest levels of UA activity exhibited better profile of calcium- and oxidative stress-driven modifications, including a significant decrease in the percentages of spherocytes and of 100- to 300-nm-sized vesicles, typically associated with the exovesiculation of stored RBCs.
The antioxidant activity of UA is associated with donor-specific differences in the performance of RBCs under storage in nonleukoreduced CPDA units.
先前对甘露醇添加剂溶液中白细胞滤除的红细胞（RBC）单位进行的研究表明，体内尿酸（UA）水平与RBC对储存损伤标志物的易感性密切相关。在本研究中，我们检验了在不同条件下，即CPDA-1中且无白细胞滤除时，UA与RBC应对储存氧化应激的能力是否具有相似的相关性。
在CPDA中未进行白细胞滤除的RBC单位（n = 47）中测量上清液的UA依赖性抗氧化能力。通过监测RBC和上清液的几种生理特性，包括细胞形状、活性氧和细胞外囊泡的大小分布，评估UA变异性对储存损伤特征的可能影响，这些生理特性来自供体中UA活性最低或最高水平的单位（n = 16），贯穿整个储存期。
在储存的RBC单位中，上清液的UA依赖性抗氧化活性随储存时间而下降，但始终与新鲜血液中的UA水平密切相关。与UA活性低的单位相反，UA活性最高的RBC表现出更好的钙和氧化应激驱动修饰特征，包括球形红细胞和100至300纳米大小囊泡的百分比显著降低，这些通常与储存RBC的外囊泡形成有关。
在未进行白细胞滤除的CPDA单位中储存时，UA的抗氧化活性与供体特异性RBC性能差异有关。
