Lisenko Katharina, Pavel Petra, Bruckner Thomas, Puthenparambil Joe, Hundemer Michael, Schmitt Anita, Witzens-Harig Mathias, Ho Anthony D, Wuchter Patrick
Department of Medicine V, Heidelberg University, Heidelberg, Germany.
Stem Cell Laboratory, IKTZ Heidelberg GmbH, Heidelberg, Germany.
J Clin Apher. 2017 Feb;32(1):27-34. doi: 10.1002/jca.21463. Epub 2016 Apr 20.
Terumo BCT recently introduced a new system for mononuclear cell (MNC) collection that uses a Spectra Optia apheresis machine equipped with a redesigned disposable kit and software program (version 11.2). It allows for the continuous collection of MNCs, unlike the original Spectra Optia system (version 7.2), which included a chamber for two-step cell separation. The aim of this study was to compare the two apheresis systems in regard to specific performance parameters. A retrospective data analysis of 150 patients who had undergone peripheral blood stem cell collection between March of 2014 and May of 2015 at our institution was performed. For the matched comparison, patients were divided into two groups by diagnosis and by previous forms of therapy received: a homogeneous group of patients with multiple myeloma (MM) that had received first line therapy ("MM" group, n = 88) and a heterogeneous group that included all of the other patients ("other" group, n = 62). No significant differences in CD34+ collection yields between both collection regimens were found (p = 0.19, p = 0.74) in either group. Moreover, similar performance ratios (collected/predicted CD34+ cell number in %) were observed (p = 0.89, p = 0.1). No relevant variations in platelet or hemoglobin loss were found between the two systems. We conclude that the new continuous Spectra Optia MNC system is equally efficient in collecting CD34+ cells and can be used without sacrificing collection efficiency levels when treating a broad variety of autologous patients. J. Clin. Apheresis 32:27-34, 2017. © 2016 Wiley Periodicals, Inc.
泰尔茂血液系统公司（Terumo BCT）最近推出了一种用于单核细胞（MNC）采集的新系统，该系统使用配备了重新设计的一次性套件和软件程序（版本11.2）的光谱Optia血细胞分离机。与最初的光谱Optia系统（版本7.2）不同，它允许连续采集MNC，最初的系统包括一个用于两步细胞分离的腔室。本研究的目的是比较这两种血细胞分离系统的特定性能参数。对2014年3月至2015年5月在我们机构接受外周血干细胞采集的150例患者进行了回顾性数据分析。为了进行匹配比较，根据诊断和先前接受的治疗形式将患者分为两组：接受一线治疗的多发性骨髓瘤（MM）同质患者组（“MM”组，n = 88）和包括所有其他患者的异质组（“其他”组，n = 62）。在两组中，两种采集方案之间的CD34 +采集产量均未发现显著差异（p = 0.19，p = 0.74）。此外，观察到相似的性能比率（采集的/预测的CD34 +细胞数百分比）（p = 0.89，p = 0.1）。在两个系统之间未发现血小板或血红蛋白损失的相关差异。我们得出结论，新的连续光谱Optia MNC系统在采集CD34 +细胞方面同样高效，并且在治疗各种自体患者时可以在不牺牲采集效率水平的情况下使用。《临床血液成分单采杂志》32:27 - 34，2017年。© 2016威利期刊公司。
