疫苗佐剂人参皂苷Rg1增强小鼠对乙型肝炎表面抗原的免疫反应。
Vaccine adjuvant ginsenoside Rg1 enhances immune responses against hepatitis B surface antigen in mice.
作者信息
Yuan Ding, Yuan Qin, Cui Qianqian, Liu Chaoqi, Zhou Zhiyong, Zhao Haixia, Dun Yaoyan, Wang Ting, Zhang Changcheng
机构信息
a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
b Renhe Hospital, The Second College of Clinical Medical Science, Three Gorges University, Yichang, Hubei 443001, China.
出版信息
Can J Physiol Pharmacol. 2016 Jun;94(6):676-81. doi: 10.1139/cjpp-2015-0528. Epub 2016 Mar 4.
The adjuvant effect of ginsenoside Rg1 on immune responses against hepatitis B surface antigen (HBsAg) in mice was investigated. Female BALB/c mice were subcutaneously injected with saline or HBsAg antigen with or without Rg1 on days 7 and 21. Samples were collected 2 weeks after the boosting for the detection of anti-HBsAg immunoglobulin G (IgG) isotypes in sera and gamma interferon (IFN-γ) and interleukin-4 (IL-4) produced in splenocytes. The innate and adaptive immune responses were measured in mice immunized as described above. The results showed that ginsenoside Rg1 had adjuvant properties in stimulating IgG, splenocyte proliferation, and mRNA expression of cytokines IFN-γ and IL-4, as well as the expression of cell surface marker TLR4 in the HBsAg-immunized mice. These results indicate that Rg1 enhances both Th1 (IgG2b and IFN-γ) and Th2 (IgG1 and IL-4) responses. In addition, the TLR4 signaling pathway is involved in the adjuvant activities of ginsenoside Rg1.
研究了人参皂苷Rg1对小鼠抗乙型肝炎表面抗原（HBsAg）免疫反应的佐剂作用。在第7天和第21天，对雌性BALB/c小鼠皮下注射生理盐水或含或不含Rg1的HBsAg抗原。加强免疫2周后收集样本，用于检测血清中抗HBsAg免疫球蛋白G（IgG）亚型以及脾细胞产生的γ干扰素（IFN-γ）和白细胞介素-4（IL-4）。对按上述方法免疫的小鼠的固有免疫和适应性免疫反应进行了检测。结果表明，人参皂苷Rg1在刺激IgG、脾细胞增殖、细胞因子IFN-γ和IL-4的mRNA表达以及HBsAg免疫小鼠中细胞表面标志物TLR4的表达方面具有佐剂特性。这些结果表明，Rg1增强了Th1（IgG2b和IFN-γ）和Th2（IgG1和IL-4）反应。此外TLR4信号通路参与了人参皂苷Rg1的佐剂活性。
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