a Dipartimento di Scienze Mediche Traslazionali , Università di Napoli Federico II , Napoli , Italy.
Expert Rev Mol Diagn. 2016 Jul;16(7):723-32. doi: 10.1080/14737159.2016.1179115. Epub 2016 May 6.
Laboratory diagnosis of HIV infection is essential for the prevention of infection and the identification of infected individuals who could benefit from highly active antiretroviral therapy. Since the release of the first assays for the detection of anti-HIV antibodies, the technology of immunoassays has improved.
Fourth generation assays - simultaneously detecting HIV p24 antigen and antibodies - have been developed and have been a major improvement in the detection of HIV infection, with a reduction of the diagnostic window. Studies have provided definite evidence for their clinical utility. Combination assays with separate results for anti-HIV antibodies and p24 antigen have been developed. Expert Commentary: In conclusion, fourth generation assays are an effective tool for the laboratory diagnosis of HIV infection. The ADVIA Centaur HIV Ag/Ab Combo assay is in line with most recent fourth generation assays and its clinical utility has been assessed.
HIV 感染的实验室诊断对于预防感染和识别可能受益于高效抗逆转录病毒治疗的感染者至关重要。自首次检测抗 HIV 抗体的检测方法问世以来，免疫分析技术得到了改进。
已开发出第四代检测方法——同时检测 HIV p24 抗原和抗体——这是 HIV 感染检测的重大改进，可缩短诊断窗口期。研究为其临床应用提供了明确的证据。已开发出具有单独抗 HIV 抗体和 p24 抗原检测结果的组合检测方法。
总之，第四代检测方法是 HIV 感染实验室诊断的有效工具。ADVIA Centaur HIV Ag/Ab Combo 检测方法符合最新的第四代检测方法，其临床应用已得到评估。
