Lyashchenko Konstantin P, Greenwald Rena, Sikar-Gang Alina, Sridhara Archana A, Johnathan Ashley, Lambotte Paul, Esfandiari Javan, Maggioli Mayara F, Thacker Tyler C, Palmer Mitchell V, Waters W Ray
Chembio Diagnostic Systems, Inc., Medford, New York, USA
Chembio Diagnostic Systems, Inc., Medford, New York, USA.
Clin Vaccine Immunol. 2017 Jun 5;24(6). doi: 10.1128/CVI.00069-17. Print 2017 Jun.
The presence of circulating antigen in cattle experimentally infected with was demonstrated using dual-path platform (DPP) technology. The antigen capture immunoassays employed rabbit polyclonal antibody recognizing predominantly complex-specific epitopes and were able to detect soluble substances and whole cells of mycobacteria. The antigen found in serum appeared to be mostly bound to IgM, but not to IgG, within the immune complexes formed at early stages of infection. The antigen was also detected in bile and urine, indicating possible clearance pathways. The data correlation analyses supported the idea of the role of IgM responses in antigen persistence during infection. The antigen was detectable in serum months prior to detectable antibody seroconversion. This proof-of-concept study suggested the potential for improved immunodiagnostics for bovine tuberculosis.
利用双路径平台（DPP）技术证实了在实验感染牛的体内存在循环抗原。抗原捕获免疫测定采用主要识别复合物特异性表位的兔多克隆抗体，能够检测分枝杆菌的可溶性物质和全细胞。在感染早期形成的免疫复合物中，血清中发现的抗原似乎大多与IgM结合，而不与IgG结合。在胆汁和尿液中也检测到了该抗原，表明可能存在清除途径。数据相关性分析支持了IgM反应在感染期间抗原持续存在中所起作用的观点。在可检测到抗体血清转化前几个月就能在血清中检测到该抗原。这项概念验证研究表明了改进牛结核病免疫诊断方法的潜力。
