Yakhchali Mohammad, Bahramnejad Kia
Dep. of Pathobiology, Parasitology division, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Iran J Parasitol. 2016 Jan-Mar;11(1):30-4.
Fasciolosis, caused by the liver flukes of the genus Fasciola, is one of the most prevalent diseases of domestic livestock and human throughout the world, imposing considerable economic losses. The present study was aimed to assess the effects of different pH values on hatching rate of Fasciola miracidia.
The flukes were isolated from the infected livers of the slaughtered ruminants at the abattoir of Urmia City, Iran, crushed thoroughly and sieved for isolation of the Fasciola eggs. The eggs were washed up several times by PBS (0.01N, pH 7.2). They were incubated at different pH values of 7±0.1 (control) and 3-9.5 (treatments) at 28°C for 16 days.
The maximum hatching rate was observed at pH 7 (14.93±0.65%), while no miracidia were hatched at pH 3 and/or pH 9-9.5. There were significant differences between the hatching rate of the treatments and that of the control group.
Water pH is proven to be a crucial factor affecting the life cycle of Fasciola and its epidemiology.
由片形吸虫属肝吸虫引起的片形吸虫病是全世界家畜和人类中最普遍的疾病之一，造成了相当大的经济损失。本研究旨在评估不同pH值对片形吸虫毛蚴孵化率的影响。
吸虫从伊朗乌尔米亚市屠宰场被感染的反刍动物肝脏中分离出来，彻底碾碎并过筛以分离片形吸虫卵。虫卵用PBS（0.01N，pH 7.2）洗涤数次。它们在28°C下于7±0.1（对照）和3 - 9.5（处理组）的不同pH值下孵育16天。
在pH 7时观察到最高孵化率（14.93±0.65%），而在pH 3和/或pH 9 - 9.5时未孵化出毛蚴。处理组的孵化率与对照组之间存在显著差异。
已证明水体pH是影响片形吸虫生命周期及其流行病学的关键因素。
