Georgieva Katya, Georgieva Simona, Mizinska Yana, Stoitsova Stoyanka R
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Acta Parasitol. 2012 Mar;57(1):46-52. doi: 10.2478/s11686-012-0007-8. Epub 2012 Feb 25.
The lectin binding properties of Fasciola hepatica miracidia were studied by a panel of fluorescein- and gold-conjugated lectins (ConA, LCA, WGA, LEA, SBA, HPA and UEA-I). The presence of mannose and/or glucose residues was demonstrated with ConA and LCA as weak diffuse fluorescence of the miracidial surface, which was more intense at the anterior part of the larva. The N-acetylglucosamine-binding lectins WGA and LEA reacted intensely with the whole miracidial surface. No labelling with N-acetylgalactosamine and/or galactose-specific (SBA and HPA) and fucose-specific UEA-I lectins was observed. The possibility that the specific recognition of the miracidial surface carbohydrates by lectins may initiate the process of transformation of the miracidia into sporocysts was examined in vitro in physiological saline for Galba truncatula. Incubation in the presence of ConA and WGA resulted in facilitation of the transformation process. Facilitation was absent in the presence of inhibitor sugars. Incubation in the presence of SBA or UEA-I had no effect. The results suggested a possible impact of carbohydrate-lectin interactions in transformation of miracidia of F. hepatica to sporocysts in vivo.
利用一组荧光素和金标记的凝集素（伴刀豆球蛋白A、扁豆凝集素、小麦胚凝集素、荆豆凝集素、大豆凝集素、花生凝集素和荆豆凝集素I）研究了肝片吸虫毛蚴的凝集素结合特性。伴刀豆球蛋白A和扁豆凝集素显示甘露糖和/或葡萄糖残基的存在，表现为毛蚴表面微弱的弥漫性荧光，在幼虫前部更强烈。与N-乙酰葡糖胺结合的凝集素小麦胚凝集素和荆豆凝集素与整个毛蚴表面强烈反应。未观察到与N-乙酰半乳糖胺和/或半乳糖特异性（大豆凝集素和花生凝集素）以及岩藻糖特异性荆豆凝集素I的标记。在截口圆扁螺的生理盐水中体外研究了凝集素对毛蚴表面碳水化合物的特异性识别是否可能启动毛蚴转化为子孢蚴的过程。在伴刀豆球蛋白A和小麦胚凝集素存在下孵育促进了转化过程。在存在抑制糖的情况下则不存在促进作用。在大豆凝集素或荆豆凝集素I存在下孵育没有影响。结果表明碳水化合物-凝集素相互作用可能对肝片吸虫毛蚴在体内转化为子孢蚴有影响。
