Szpon Łukasz, Stal Aleksander, Zawadzki Marcin, Lis-Nawara Anna, Kielan Wojciech, Grzebieniak Zygmunt
Pol Przegl Chir. 2016 Jan 1;88(1):15-9. doi: 10.1515/pjs-2016-0021.
Due to increased colorectal cancer incidence there is a necessity of seeking new both prognostic and prediction factors that will allow to evolve new diagnostic tests. K-ras gene seems to be such a factor and its mutations are considered to be an early marker of progression of colorectal cancer. The aim of the study was to find a correlation between K-ras gene mutation in patients with diagnosed colorectal cancer and selected clinical parameters.
A total of 104 patients (41 women and 63 men) with diagnosed colorectal cancer were included in this study. The average age of male group was 68.3 and in female group - 65.9. Samples were taken from paraffine blocks with tissue from diagnosed patients and K-ras gene mutation were identified. Afterwards the statistical analysis was made seeking the correlation between K-ras gene mutation incidence and clinical TNM staging system, tumour localisation, histological type, sex, age.
K-ras gene mutations were detected in 20.1% of all colorectal cancers. Significantly higher rate of K-ras gene mutations were diagnosed among patients classified at stage I (40%), stage IIC (50%) and stage IV (50%) according to the TNM classification.
The results of our study are compatible with other studies and indicate the correlation between K-ras gene mutation and colorectal cancer incidence. Identification of K-ras gene mutation may complement other diagnostic methods at early stage of colorectal cancer.
由于结直肠癌发病率上升，有必要寻找新的预后和预测因素，以开发新的诊断测试。K-ras基因似乎就是这样一个因素，其突变被认为是结直肠癌进展的早期标志物。本研究的目的是找出已确诊结直肠癌患者的K-ras基因突变与选定临床参数之间的相关性。
本研究共纳入104例已确诊结直肠癌的患者（41例女性和63例男性）。男性组的平均年龄为68.3岁，女性组为65.9岁。从确诊患者的石蜡块组织中取样，鉴定K-ras基因突变。随后进行统计分析，寻找K-ras基因突变发生率与临床TNM分期系统、肿瘤定位、组织学类型、性别、年龄之间的相关性。
在所有结直肠癌中，20.1%检测到K-ras基因突变。根据TNM分类，在I期（40%）、IIC期（50%）和IV期（50%）患者中，K-ras基因突变的诊断率显著更高。
我们的研究结果与其他研究一致，表明K-ras基因突变与结直肠癌发病率之间存在相关性。K-ras基因突变的鉴定可能在结直肠癌早期补充其他诊断方法。
