[上海市综合医院腹泻患者溶组织内阿米巴感染情况调查]

[Investigation on Entamoeba histolytica infection in diarrhea patients from general hospitals in Shanghai City].

作者信息

Zhang Xiao-ping, He Yan-yan, Wang Zhen-yu, Zhang Yao-guang, Zhu Qian, Jiang Shou-fu, Li Ying, Cheng Yu-ping, Yin Ming-min

出版信息

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2015 Dec;27(6):600-3.

PMID:27097477

Abstract

OBJECTIVE

To understand the status of Entamoeba histolytica infection in diarrhea patients in general hospitals, so as to provide the evidences for the prevention and control of the disease.

METHODS

The diarrhea patients in intestinal disease clinics of 3 general hospitals in Shanghai City were chosen as the investigation objectives, and their fecal and blood samples were collected, and then were detected by the normal saline direct smear method and iodine solution staining, immunochromatographic method and ELISA respectively to understand the infection status of E. histolytica, and the characteristics of the infected persons were analyzed. RESULTS Totally 1 015 fecal samples were detected, and among which 36 positive ones were detected by parasitological examinations, with a general positive rate of 3.55%. There were no statistically significant differences among the positive rates of patients from the three hospitals (P > 0.05), nor between or among those of the patients with different sexes, ages, occupations and education levels (all P > 0.05). The positive rate of E. histolytica in bloody purulent stools was higher than those in loose stools and watery stools (both P < 0.01). The peak period of infection was from July to September. Among the 36 infected people detected by parasitological examination, 88.90% of them complained about abdominal pain, and the red blood cells and leucocyte cells were found in the stool samples of 75.00% and 22.23% of the cases, respectively. The positive rates of E. histolytica were 8.18% (83/1 015) and 7.12% (48/675) respectively when detected by the immunochromatographic method and ELISA.

CONCLUSIONS

Summer and autumn are the high risk seasons for E. histolytica infection, and the surveillance should be strengthened in this period. The positive rate of E. histolytica in samples of bloody purulent stools is high, and the combined application of several detection methods can increase the detection rate.

摘要

目的

了解综合医院腹泻患者溶组织内阿米巴感染状况，为该病的防控提供依据。

方法

选取上海市3家综合医院肠道门诊腹泻患者作为调查对象，采集其粪便及血液样本，分别采用生理盐水直接涂片法、碘液染色法、免疫层析法及酶联免疫吸附测定法进行检测，了解溶组织内阿米巴感染情况，并分析感染者特征。结果共检测粪便样本1 015份，其中寄生虫学检查阳性36份，总阳性率为3.55%。3家医院患者的阳性率差异无统计学意义（P>0.05），不同性别、年龄、职业及文化程度患者的阳性率差异也均无统计学意义（均P>0.05）。脓血便中溶组织内阿米巴的阳性率高于稀便和水样便（均P<0.01）。感染高峰期为7至9月。寄生虫学检查检出的36例感染者中，88.90%有腹痛症状，75.00%的病例粪便样本中发现红细胞，22.23%的病例粪便样本中发现白细胞。免疫层析法和酶联免疫吸附测定法检测溶组织内阿米巴的阳性率分别为8.18%（83/1 015）和7.12%（48/675）。