[Research on dust mite allergen gathered from filters of air-conditioners].

OBJECTIVE

To discuss the relation between the dust mite allergen (Der) in air-conditioner filters and the asthma attack.

METHODS

The dust samples were collected from the filters of air-conditioners in dining rooms, shopping malls, hotels and households, respectively. The concentrations of Der f 1 and Der p1 were detected by ELISA, and the dust mite immune activities were determined by dot-ELISA.

RESULTS

The concentrations of Der f 1 in the dining rooms, shopping malls, hotels and households were 1.52, 1.24, 1.31 µg/g and 1.46 µg/g respectively, and the concentrations of Der p 1 were 1.23, 1.12, 1.16 µg/ g and 1.18 µg, respectively. One hour after the running of air-conditioners, the concentrations of Der f 1 and Der p 1 in the air were higher than those before the running of air-conditioners, and the differences were significant (P < 0.05). Additionally, the dot-ELISA results revealed that the allergen extracted from the dust was capable of reacting with IgE from the sera of asthma mice allergic to dust mites.

CONCLUSION

Der f 1 and Der p 1 appear abundantly in the filters of air-conditioners in domestic houses in Wuhu City, and the allergens can induce asthma.