中国南方海口地区哮喘儿童的室内尘螨过敏原水平、特异性 IgE 流行率和 IgE 交叉抑制模式。

Indoor mite allergen levels, specific IgE prevalence and IgE cross-inhibition pattern among asthmatic children in Haikou, southern China.

机构信息

Research Asia Pacific, ALK A/S, Guangzhou, Guangdong 510230, China.

出版信息

Chin Med J (Engl). 2012 Sep;125(17):3059-63.

Abstract

BACKGROUND

Haikou locates in tropical island with unique mite propagation. The aim of this stuy is to determine mite allergens levels in Haikou, and to investigate the prevalence of mite specific IgE-sensitization and IgE cross-reactivity between house dust mites.

METHODS

Allergen and antigen concentrations against six mite species were tested by enzyme-linked immunosorbent assay (ELISA). Specific IgE concentrations and cross-inhibitions were measured with ADVIA Centaur(®).

RESULTS

Allergen or antigen Dermatophagoides pteronyssinus (Der p 1), Blomia tropicalis (Blo t) and Tyrophagus putrescentia (Tyr p) were detected in dust samples. Dermatophagoides farinae (Der f 1), Lepidoglyphus destructor (Lep d 2), and Acarus siro (Aca s) were found in very few samples. Specific IgE tests showed high prevalence of sensitizations against all tested mites with high IgE levels to Der p, Der f, and Blo t. Storage mites, Blo t, Tyr p, Lep d, and Aca s, could inhibit Der p from 0 to 50%. Storage mites could inhibit Der f between 30% and 100%. Der p IgE could be inhibited by Der f with up to 90%, and vice versa. Der p could inhibit Blo t from 40% to 80%. Blo t was able to fully inhibit IgE binding to Lep d, Tyr p, and Aca s compared to partial inhibition by Der p.

CONCLUSIONS

Der p is the dominating mite and has the highest specific IgE prevalence among asthmatic children. Blo t represents an important source of storage mite sensitization and some patients may be independently sensitized to both Der p and Blo t. High prevalence of sensitization to Der f may be due to IgE-mediated cross-reactivity with Der p and Blo t.

摘要

背景

海口位于热带岛屿，拥有独特的螨虫繁殖环境。本研究旨在确定海口的螨虫过敏原水平，并研究屋尘螨特异性 IgE 致敏和 IgE 交叉反应的流行情况。

方法

通过酶联免疫吸附试验（ELISA）检测 6 种螨虫的过敏原和抗原浓度。使用 ADVIA Centaur（®）测量特异性 IgE 浓度和交叉抑制。

结果

在尘样中检测到屋尘螨（Der p 1）、热带无爪螨（Blo t）和腐食酪螨（Tyr p）的过敏原或抗原。粉尘螨（Der f 1）、粗脚粉螨（Lep d 2）和智利小植绥螨（Aca s）仅在极少数样本中发现。特异性 IgE 检测显示，所有测试螨虫的致敏率均较高，且对 Der p、Der f 和 Blo t 的 IgE 水平较高。储存螨、Blo t、Tyr p、Lep d 和 Aca s 可抑制 Der p 0 至 50%。储存螨可抑制 Der f 在 30%至 100%之间。Der p IgE 可被 Der f 抑制高达 90%，反之亦然。Der p 可抑制 Blo t 40%至 80%。Blo t 能够完全抑制 IgE 与 Lep d、Tyr p 和 Aca s 的结合，而 Der p 只能部分抑制。