Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, Moscow, Russia, 119071.
Plant Mol Biol. 2016 Jul;91(4-5):441-58. doi: 10.1007/s11103-016-0478-3. Epub 2016 Apr 20.
Myco-heterotroph Monotropa hypopitys is a widely spread perennial herb used to study symbiotic interactions and physiological mechanisms underlying the development of non-photosynthetic plant. Here, we performed, for the first time, transcriptome-wide characterization of M. hypopitys miRNA profile using high throughput Illumina sequencing. As a result of small RNA library sequencing and bioinformatic analysis, we identified 55 members belonging to 40 families of known miRNAs and 17 putative novel miRNAs unique for M. hypopitys. Computational screening revealed 206 potential mRNA targets for known miRNAs and 31 potential mRNA targets for novel miRNAs. The predicted target genes were described in Gene Ontology terms and were found to be involved in a broad range of metabolic and regulatory pathways. The identification of novel M. hypopitys-specific miRNAs, some with few target genes and low abundances, suggests their recent evolutionary origin and participation in highly specialized regulatory mechanisms fundamental for non-photosynthetic biology of M. hypopitys. This global analysis of miRNAs and their potential targets in M. hypopitys provides a framework for further investigation of miRNA role in the evolution and establishment of non-photosynthetic myco-heterotrophs.
Myco-heterotroph 植物绵马鳞毛蕨是一种广泛分布的多年生草本植物，用于研究共生相互作用和非光合植物发育的生理机制。在这里，我们首次使用高通量 Illumina 测序对绵马鳞毛蕨的 miRNA 谱进行了全转录组特征分析。通过小 RNA 文库测序和生物信息学分析，我们鉴定了 55 个属于已知 miRNA 的 40 个家族的成员和 17 个绵马鳞毛蕨特有的推定新 miRNA。计算筛选揭示了已知 miRNA 的 206 个潜在 mRNA 靶标和新 miRNA 的 31 个潜在 mRNA 靶标。预测的靶基因在基因本体论术语中进行了描述，并发现它们参与了广泛的代谢和调节途径。新的绵马鳞毛蕨特异性 miRNA 的鉴定，其中一些靶基因较少且丰度较低，表明它们是最近进化而来的，参与了非光合绵马鳞毛蕨生物学中基本的高度专业化调节机制。对绵马鳞毛蕨中 miRNAs 及其潜在靶标的全面分析为进一步研究 miRNA 在非光合 myco-heterotrophs 的进化和建立中的作用提供了框架。
