Doganci S, Yildirim V, Erol G, Yesildal F, Karabacak K, Kadan M, Ozkan G, Ince M E, Ozgurtas T
Department of Cardiovascular Surgery, Gulhane Military Medical Academy, Ankara, Turkey.
Eur Rev Med Pharmacol Sci. 2016 Apr;20(7):1384-9.
Polidocanol is the most frequently used sclerosant for sclerotherapy all around the world. Our experimental research aims to find out the angiogenic effects of Polidocanol.
Angiogenic activity of polidocanol was examined in vivo in the chick chorioallantoic membrane (CAM) model, cell viability assay (human umbilical vein endothelial cells - HUVECs) and in vitro tube formation assay of HUVECs.
In CAM assay, a significant decrease on CAM vessel growth was observed after the application of polidocanol solutions. Vessel growth inhibition was strongly dose-dependent. There was a cytotoxic effect on HUVECs in the presence of polidocanol observed with MTT assay (p < 0.05). In the tube formation assay, statistically significant decrease in tube formation was observed in polidocanol group. It was found that polidocanol had an anti-angiogenic effect (p < 0.05). The results provide evidence that polidocanol decreases angiogenesis and has a cytotoxic effect on ECs.
These results provide evidence that Polidocanol (lauromacrogol 400) have strong anti-angiogenic effects in vitro and in vivo. Further researches needed to reveal early and long-term effects of polidocanol in the human vascular system and new treatment approach as an anti-angiogenic therapy.
聚多卡醇是全球最常用于硬化治疗的硬化剂。我们的实验研究旨在探究聚多卡醇的血管生成作用。
在鸡胚绒毛尿囊膜（CAM）模型中对聚多卡醇的血管生成活性进行体内研究，采用细胞活力测定法（人脐静脉内皮细胞 - HUVECs）以及HUVECs的体外管腔形成测定法。
在CAM测定中，应用聚多卡醇溶液后观察到CAM血管生长显著减少。血管生长抑制呈强烈的剂量依赖性。MTT测定显示在聚多卡醇存在下对HUVECs有细胞毒性作用（p < 0.05）。在管腔形成测定中，聚多卡醇组的管腔形成有统计学意义的显著减少。发现聚多卡醇具有抗血管生成作用（p < 0.05）。结果表明聚多卡醇可减少血管生成并对内皮细胞有细胞毒性作用。
这些结果表明聚多卡醇（聚乙二醇月桂醚400）在体外和体内均具有强大的抗血管生成作用。需要进一步研究以揭示聚多卡醇在人体血管系统中的早期和长期作用以及作为抗血管生成疗法的新治疗方法。
