Heuke Sandro, Chernavskaia Olga, Bocklitz Thomas, Legesse Fisseha Bekele, Meyer Tobias, Akimov Denis, Dirsch Olaf, Ernst Günther, von Eggeling Ferdinand, Petersen Iver, Guntinas-Lichius Orlando, Schmitt Michael, Popp Jürgen
Leibniz Institute of Photonic Technology, Jena, Germany.
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University, Jena, Germany.
Head Neck. 2016 Oct;38(10):1545-52. doi: 10.1002/hed.24477. Epub 2016 Apr 21.
Treatment of early cancer stages is deeply connected to a good prognosis, a moderate reduction of the quality of life, and comparably low treatment costs.
Head and neck squamous cell carcinomas were investigated using the multimodal combination of coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence (TPEF), and second-harmonic generation (SHG) microscopy.
An increased median TPEF to CARS contrast was found comparing cancerous and healthy squamous epithelium with a p value of 1.8·10(-10) . A following comprehensive image analysis was able to predict the diagnosis of imaged tissue sections with an overall accuracy of 90% for a 4-class model.
Nonlinear multimodal imaging is verified objectively as a valuable diagnostic tool that complements conventional staining protocols and can serve as filter in future clinical routine reducing the pathologist's workload. © 2016 Wiley Periodicals, Inc. Head Neck 38: First-1552, 2016.
早期癌症阶段的治疗与良好的预后、生活质量的适度降低以及相对较低的治疗成本密切相关。
使用相干反斯托克斯拉曼散射（CARS）、双光子激发荧光（TPEF）和二次谐波产生（SHG）显微镜的多模态组合对头颈部鳞状细胞癌进行研究。
比较癌性和健康鳞状上皮时，发现TPEF与CARS对比度的中位数增加，p值为1.8·10（-10）。随后的综合图像分析能够预测成像组织切片的诊断，对于四分类模型，总体准确率为90%。
非线性多模态成像被客观地验证为一种有价值的诊断工具，它补充了传统染色方案，并可在未来临床常规中作为过滤器，减轻病理学家的工作量。©2016威利期刊公司。头颈38：First-1552，2016。
