哥伦比亚甙元通过诱导HCT116结肠癌细胞凋亡和坏死性凋亡来抑制细胞增殖。
Columbianadin Inhibits Cell Proliferation by Inducing Apoptosis and Necroptosis in HCT116 Colon Cancer Cells.
作者信息
Kang Ji In, Hong Ji-Young, Choi Jae Sue, Lee Sang Kook
机构信息
College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
Department of Food Science and Nutrition, Pukyong National University, Busan 46241, Republic of Korea.
出版信息
Biomol Ther (Seoul). 2016 May 1;24(3):320-7. doi: 10.4062/biomolther.2015.145.
Columbianadin (CBN), a natural coumarin from Angelica decursiva (Umbelliferae), is known to have various biological activities including anti-inflammatory and anti-cancer effects. In this study, the anti-proliferative mechanism of actions mediated by CBN was investigated in HCT-116 human colon cancer cells. CBN effectively suppressed the growth of colon cancer cells. Low concentration (up to 25 µM) of CBN induced apoptosis, and high concentration (50 µM) of CBN induced necroptosis. The induction of apoptosis by CBN was correlated with the modulation of caspase-9, caspase-3, Bax, Bcl-2, Bim and Bid, and the induction of necroptosis was related with RIP-3, and caspase-8. In addition, CBN induced the accumulation of ROS and imbalance in the intracellular antioxidant enzymes such as SOD-1, SOD-2, catalase and GPx-1. These findings demonstrate that CBN has the potential to be a candidate in the development of anti-cancer agent derived from natural products.
蛇床子素（CBN）是一种从独活（伞形科）中提取的天然香豆素，已知具有多种生物活性，包括抗炎和抗癌作用。在本研究中，研究了CBN介导的抗增殖作用机制在HCT-116人结肠癌细胞中的情况。CBN有效地抑制了结肠癌细胞的生长。低浓度（高达25μM）的CBN诱导细胞凋亡，高浓度（50μM）的CBN诱导坏死性凋亡。CBN诱导的细胞凋亡与caspase-9、caspase-3、Bax、Bcl-2、Bim和Bid的调节相关，而坏死性凋亡的诱导与RIP-3和caspase-8有关。此外，CBN诱导活性氧的积累以及细胞内抗氧化酶如SOD-1、SOD-2、过氧化氢酶和GPx-1的失衡。这些发现表明，CBN有潜力成为天然产物来源抗癌药物开发的候选物。
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