Adisa Akinyele O, Udeabor Samuel E, Kubesch Alica, Barbeck Mike, Ghanaati Shahram
Department of Oral, Cranio Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main; REPAIR Lab, Institute of Pathology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
Niger Postgrad Med J. 2016 Jan-Mar;23(1):44-6. doi: 10.4103/1117-1936.180187.
It is occasionally difficult to distinguish the stellate reticulum-like region of ameloblastoma from the fibrous connective tissue stroma. This difficulty is further pronounced in the plexiform variant of ameloblastoma that has very sparse fibrous connective tissue.
To test the utility of Azan trichrome stain in marking tumour regions and the peri-tumour environment of ameloblastoma.
Sections were prepared for 18 formalin fixed paraffin-embedded blocks of ameloblastoma cases and stained with Azan trichrome stain according to the manufacturer's specification.
The tumour areas were stained mostly brown, with the ameloblasts mainly marked as deep brown while the stellate reticulum-like region was light brown. The structures in the peri-tumour region were marked with different shades of blue. Azan trichrome staining was able to distinguish between the fibrous connective tissue and the stellate reticulum-like areas in 100% of the cases.
偶尔很难将成釉细胞瘤的星网状样区域与纤维结缔组织基质区分开来。在纤维结缔组织非常稀疏的丛状型成釉细胞瘤中，这种困难更加明显。
测试偶氮三色染色在标记成釉细胞瘤的肿瘤区域和肿瘤周围环境中的效用。
制备18例成釉细胞瘤病例的福尔马林固定石蜡包埋块切片，并根据制造商的说明书用偶氮三色染色法进行染色。
肿瘤区域大多染成棕色，成釉细胞主要标记为深棕色，而星网状样区域为浅棕色。肿瘤周围区域的结构用不同深浅的蓝色标记。偶氮三色染色在100%的病例中能够区分纤维结缔组织和星网状样区域。
