Department of Oral Medicine and Diagnostic Science Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
J Oral Pathol Med. 2013 Feb;42(2):186-93. doi: 10.1111/j.1600-0714.2012.01195.x. Epub 2012 Jul 2.
The altered expression of syndecan-1 (SD-1), a transmembrane heparan sulfate proteoglycan, in ameloblastomas and cysts of odontogenic origin suggests that this molecule could have prognostic value in assessing the clinical outcome of those lesions. The purpose of this study was to analyze SD-1 expression profile immunohistochemically in archival, paraffin-embedded tissue sections of ameloblastomas and in common odontogenic cysts arising from the same locale.
SD-1 expression was investigated in 32 ameloblastomas, 26 keratocystic odontogenic tumors (KCOT), and 21 dentigerous cysts from the archives of the histopathology laboratory which were routinely processed. The cases were reviewed and assessed according to the established criteria. Sections were immunostained with monoclonal antibody against SD-1 (CD138). Sections of normal oral mucosa, site matched, were stained in parallel as positive controls. The plasma cells in sections served as internal positive controls.
SD-1 expression was observed in the epithelial and stromal elements of the sections, and the expression was significantly associated with the lesion's extension and involvement of adjacent structures (P=0.025). Stellate-reticulum cells showed higher expression than the ameloblasts, which was at a significant level (P<0.0001). Highly significant difference was reported among the three groups of lesion for the epithelial staining (P<0.0001). The mean rank scores (Kruskal-Wallis test) of ameloblastomas were significantly lower than those of KCOT and dentigerous cysts. Non-significant comparison was made between KCOT and dentigerous cyst groups.
The present study revealed SD-1 immunoreactivity in the stromal cells of ameloblastoma, KCOT, and dentigerous cysts rather uniformly. This reported SD-1 expression by the tumor stroma is considered to be associated with poor prognosis of the lesions.
硫酸乙酰肝素蛋白聚糖-1（SD-1）在牙源性造釉细胞瘤和囊肿中的表达改变表明，该分子在评估这些病变的临床结果方面可能具有预后价值。本研究的目的是分析 SD-1 在造釉细胞瘤和同一部位发生的常见牙源性囊肿的存档石蜡包埋组织切片中的免疫组织化学表达谱。
对组织病理学实验室存档的 32 例造釉细胞瘤、26 例角化囊肿性牙源性肿瘤（KCOT）和 21 例含牙囊肿进行 SD-1 表达检测。对病例进行回顾性评估，并根据既定标准进行评估。使用针对 SD-1（CD138）的单克隆抗体对切片进行免疫染色。平行染色来自同一部位的正常口腔黏膜切片作为阳性对照。浆细胞作为内部阳性对照。
在切片的上皮和基质成分中观察到 SD-1 表达，表达与病变的扩展和邻近结构的受累显著相关（P=0.025）。星状网状细胞的表达高于造釉细胞，差异具有统计学意义（P<0.0001）。三组病变的上皮染色有显著差异（P<0.0001）。造釉细胞瘤的平均秩评分（Kruskal-Wallis 检验）明显低于 KCOT 和含牙囊肿。KCOT 和含牙囊肿组之间的比较无统计学意义。
本研究表明 SD-1 在造釉细胞瘤、KCOT 和含牙囊肿的基质细胞中具有免疫反应性，且表达较为均匀。报道的肿瘤基质中的 SD-1 表达被认为与病变的不良预后相关。
