Liu Xiao-Ran, Wang Yue-Ying, Fan Hai-Rui, Wu Can-Jie, Kumar Ashok, Yang Li-Guo
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Asian J Androl. 2016 Nov-Dec;18(6):920-924. doi: 10.4103/1008-682X.173449.
β-cryptoxanthin (CRY), a major carotenoid of potential interest for health, is obtained naturally from orange vegetables and fruits. A few research studies have reported that CRY could decrease oxidative stress and germ cell apoptosis. The purpose of this study was to examine the effects of CRY on acute cadmium chloride (CdCl 2 )-induced oxidative damage in rat testes. For this study, 24 rats were divided into four groups, one of which serves as a control group that received intraperitoneal (i.p.) injections of corn oil and physiological saline. The other rats were i.p. injected with CRY (10 μg kg-1 ) every 8 h, beginning 8 h before CdCl 2 (2.0 mg kg-1 ) treatment. The pathological and TUNEL findings revealed that CRY ameliorated the Cd-induced testicular histological changes and germ cell apoptosis in the rats. Furthermore, the Cd-induced decrease in the testicular testosterone (T) level was attenuated after CRY administration (P < 0.05). The administration of CRY significantly reversed the Cd-induced increases in the lipid peroxide (LPO) and malondialdehyde (MDA) levels (P < 0.01). The testicular antioxidants superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) were decreased by treatment with Cd alone but were restored by CRY co-treatment. These results demonstrated that the application of CRY can enhance the tolerance of rats to Cd-induced oxidative damage and suggest that it has promised as a pharmacological agent to protect against Cd-induced testicular toxicity.
β-隐黄质（CRY）是一种对健康具有潜在益处的主要类胡萝卜素，天然存在于橙色蔬菜和水果中。一些研究报告称，CRY可降低氧化应激和生殖细胞凋亡。本研究的目的是检测CRY对急性氯化镉（CdCl₂）诱导的大鼠睾丸氧化损伤的影响。在本研究中，将24只大鼠分为四组，其中一组作为对照组，腹腔注射玉米油和生理盐水。其他大鼠在CdCl₂（2.0 mg kg⁻¹）处理前8小时开始，每8小时腹腔注射一次CRY（10 μg kg⁻¹）。病理和TUNEL检测结果显示，CRY改善了镉诱导的大鼠睾丸组织学变化和生殖细胞凋亡。此外，CRY给药后，镉诱导的睾丸睾酮（T）水平降低得到缓解（P < 0.05）。CRY给药显著逆转了镉诱导的脂质过氧化物（LPO）和丙二醛（MDA）水平升高（P < 0.01）。单独用镉处理会降低睾丸抗氧化剂超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽（GSH）的水平，但CRY联合处理可使其恢复。这些结果表明，应用CRY可增强大鼠对镉诱导的氧化损伤的耐受性，并表明它有望作为一种预防镉诱导的睾丸毒性的药物。
