Laboratory of Bioorganic Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422–8526, Japan.
Biol Pharm Bull. 2011;34(3):311-7. doi: 10.1248/bpb.34.311.
Increased oxidative stress is known to accelerate age-related pathologies. Beta-cryptoxanthin (β-CRX, (3R)-β,β-caroten-3-ol) is a potent antioxidant that is highly rich in Satsuma mandarin orange (mandarin), which is the most popular fruit in Japan. We investigated the antioxidative and anti-aging effects of β-CRX and mandarin using senescence-accelerated mice (SAMP10), which were characterized by a short lifespan, high generation of superoxide anions in the brain and poor learning ability with aging. β-CRX (0.5-5.0 µg/ml) or mandarin juice (3.8-38.0%) was added to drinking water of SAMP10 one to 12 months of age. β-CRX was dose-dependently incorporated into the cerebral cortex and the contents were similar to the concentration of β-CRX in the human frontal lobe. These mice also had higher learning ability. The level of DNA oxidative damage was significantly lower in the cerebral cortex of mice that ingested β-CRX and mandarin than control mice. In addition, the mice that ingested β-CRX (>1.5 µg/ml) and mandarin (>11.3%) exhibited a higher survival when 12 month-old, the presenile age of SAMP10, than control mice. These results suggest that β-CRX is incorporated into the brain and has an important antioxidative role and anti-aging effect.
氧化应激的增加已知会加速与年龄相关的病理。β-隐黄质（β-CRX，（3R）-β，β-胡萝卜素-3-醇）是一种有效的抗氧化剂，在日本最受欢迎的水果温州蜜柑（蜜柑）中含量极高。我们使用加速衰老的小鼠（SAMP10）研究了β-CRX 和蜜柑的抗氧化和抗衰老作用，这些小鼠的特点是寿命短、大脑中超氧阴离子的产生高，随着年龄的增长学习能力下降。将 β-CRX（0.5-5.0µg/ml）或蜜柑汁（3.8-38.0%）添加到 SAMP10 1 至 12 月龄的饮用水中。β-CRX 呈剂量依赖性地掺入大脑皮层，其含量与人类额叶中的 β-CRX 浓度相似。这些小鼠的学习能力也更高。摄入 β-CRX 和蜜柑的小鼠大脑皮层中的 DNA 氧化损伤水平明显低于对照组小鼠。此外，摄入 β-CRX（>1.5µg/ml）和蜜柑（>11.3%）的小鼠在 12 月龄（SAMP10 的早老年龄）时的存活率高于对照组小鼠。这些结果表明，β-CRX 被掺入大脑中，具有重要的抗氧化作用和抗衰老作用。
