Azab Mohammed A, Allen Matthew J, Daniels Joshua B
Joshua B. Daniels, DVM, PhD, DACVM, The Ohio State University, College of Veterinary Medicine, 601 Vernon Tharp Dr., Columbus, OH, 43210, United States, Phone: +1 614 247 1725, Fax: +1 614 292 0895, E-mail:
Vet Comp Orthop Traumatol. 2016 Jul 19;29(4):347-50. doi: 10.3415/VCOT-15-08-0134. Epub 2016 Apr 22.
To evaluate the in vitro antibacterial activity of a silver-impregnated coating against a biofilm-forming strain of methicillin-resistant Staphylococcus pseudintermedius (MRSP).
A clinical MRSP isolate sourced from a failed canine knee implant was evaluated for biofilm production and used in the present study. Using a standard test method and a clinically approved titanium substrate, the antimicrobial activity of a novel silver plasma coating was determined at two times: five minutes after inoculation of the specimens (T0) and after 24 hours of incubation (T24). Scanning electron microscopy was used to evaluate the biofilm formation on specimens.
The tested clinical MRSP isolate was classified as a strong biofilm producer. The silver coating significantly reduced the MRSP growth more than four log steps compared to the non-coated specimens and showed more than 99.98% reduction in the number of colony forming units after 24 hours. Scanning electron microscopy images revealed that silver-coated surfaces did not manifest detectable biofilm, while biofilm formation was readily observed on the control specimens.
The silver coating exhibited excellent activity against the multidrug resistant biofilm-forming MRSP isolate. The next stage of this work will involve testing in an animal model of orthopaedic infection. Positive results from animal studies would support the introduction of the silver plasma coating as a new strategy for preventing implant contamination, biofilm formation, and surgical infection in dogs undergoing orthopaedic surgery.
评估含银涂层对耐甲氧西林中间型葡萄球菌（MRSP）生物膜形成菌株的体外抗菌活性。
对一株源自犬膝关节植入失败病例的临床MRSP分离株进行生物膜生成评估，并用于本研究。采用标准测试方法和临床认可的钛基底，在两个时间点测定新型银等离子体涂层的抗菌活性：接种标本后5分钟（T0）和孵育24小时后（T24）。使用扫描电子显微镜评估标本上的生物膜形成情况。
测试的临床MRSP分离株被归类为强生物膜产生菌。与未涂层标本相比，银涂层使MRSP生长显著降低超过四个对数级，并且在24小时后菌落形成单位数量减少超过99.98%。扫描电子显微镜图像显示，银涂层表面未观察到可检测到的生物膜，而在对照标本上很容易观察到生物膜形成。
银涂层对多药耐药的生物膜形成MRSP分离株表现出优异的活性。这项工作的下一阶段将涉及在骨科感染动物模型中进行测试。动物研究的阳性结果将支持引入银等离子体涂层作为预防接受骨科手术的犬类植入物污染、生物膜形成和手术感染的新策略。
