Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Heweliusza 1, 10-718 Olsztyn, Poland.
Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Heweliusza 1, 10-718 Olsztyn, Poland.
Bioresour Technol. 2016 Jul;212:262-270. doi: 10.1016/j.biortech.2016.04.063. Epub 2016 Apr 16.
Rape straw and Miscanthus giganteus was pretreated chemically with oxalic acid or sodium hydroxide. The pretreated substrates were hydrolyzed with enzymatic preparations of cellulase, xylanase and cellobiase. The highest concentration of reducing sugars was achieved after hydrolysis of M. giganteus pretreated with NaOH (51.53gdm(-3)). In turn, the highest yield of enzymatic hydrolysis determined based on polysaccharides content in the pretreated substrates was obtained in the experiments with M. giganteus and oxalic acid (99.3%). Rape straw and M. giganteus hydrolysates were fermented using yeast Saccharomyces cerevisiae 7, NRRL 978 or filamentous fungus Mucor rouxii (Mucor indicus) DSM 1191. The highest ethanol concentration was determined after fermentation of M. giganteus hydrolysate pretreated with NaOH using S. cerevisiae (1.92% v/v). Considering cellulose content in the pretreated solid, the highest degree of its conversion to ethanol (86.2%) was achieved after fermentation of the hydrolysate of acid-treated M. giganteus using S. cerevisiae.
稻草和芒草经草酸或氢氧化钠化学预处理后，用纤维素酶、木聚糖酶和纤维二糖酶的酶制剂进行水解。在 NaOH 预处理的芒草水解后，还原糖的最高浓度达到 51.53gdm(-3)。相反，根据预处理底物中多糖的含量，在芒草和草酸的实验中，确定了酶水解的最高产率为 99.3%。用酿酒酵母 Saccharomyces cerevisiae 7、NRRL 978 或丝状真菌毛霉（印度毛霉）DSM 1191 发酵油菜秸秆和芒草水解物。在使用 S. cerevisiae 发酵 NaOH 预处理的芒草水解物后，确定了最高乙醇浓度为 1.92%（v/v）。考虑到预处理固体中的纤维素含量，在用 S. cerevisiae 发酵酸处理的芒草水解物后，纤维素转化为乙醇的最高程度达到 86.2%。
