Nogueira-Neto Joes, Cardoso André S C, Monteiro Hugo P, Fonseca Fernando L A, Ramos Luiz Roberto, Junqueira Virginia B C, Simon Karin A
Department of Biological Sciences, Federal University of São Paulo, Diadema, SP, Brazil.
Department of Biochemistry/Molecular Biology, Federal University of São Paulo, São Paulo, SP, Brazil.
Cell Biol Int. 2016 Jul;40(7):796-802. doi: 10.1002/cbin.10618. Epub 2016 May 11.
Much attention has been drawn to the pro-inflammatory condition that accompanies aging. This study compared parameters from non-stimulated neutrophils, obtained from young (18-30 years old [y.o.]) and elderly (65-80 y.o.) human volunteers. Measured as an inflammatory marker, plasmatic concentration of hs-CRP was found higher in elderly individuals. Non-stimulated neutrophil production of ROS and NO was, respectively, 38 and 29% higher for the aged group. From the adhesion molecules evaluated, only CD11b expression was elevated in neutrophils from the aged group, whereas no differences were found for CD11a, CD18, or CD62. A 69% higher non-stimulated in vitro neutrophil/endothelial cell adhesion was observed for neutrophils isolated from elderly donors. Our results suggest that with aging, neutrophils may be constitutively producing more reactive species in closer proximity to endothelial cells of vessel walls, which may both contribute to vascular damage and reflect a neutrophil intracellular disrupted redox balance, altering neutrophil function in aging.
衰老所伴随的促炎状态已引起了广泛关注。本研究比较了从年轻（18 - 30岁）和老年（65 - 80岁）人类志愿者获取的未受刺激的中性粒细胞的参数。作为一种炎症标志物，发现老年个体的血浆hs - CRP浓度更高。老年组未受刺激的中性粒细胞产生的ROS和NO分别高出38%和29%。在所评估的黏附分子中，只有老年组中性粒细胞的CD11b表达升高，而CD11a、CD18或CD62未发现差异。从老年供体分离的中性粒细胞在体外未受刺激时与内皮细胞的黏附率高出69%。我们的结果表明，随着年龄增长，中性粒细胞可能在血管壁内皮细胞附近持续产生更多的反应性物质，这可能既导致血管损伤，又反映中性粒细胞细胞内氧化还原平衡的破坏，从而改变衰老过程中中性粒细胞的功能。
