Kitada M, Komori M, Ohi H, Imaoka S, Funae Y, Kamataki T
Division of Analytical Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.
Res Commun Chem Pathol Pharmacol. 1989 Feb;63(2):175-88.
Microsomal lipid peroxidation caused decreases in the activities of ethylmorphine N-demethylase and testosterone 6 beta-hydroxylase more markedly than aniline hydroxylase, p-phenetidine O-deethylase and testosterone 2 alpha- and 16 alpha-hydroxylases. Results of sodium dodecylsulfate-polyacrylamide gel electrophoresis of liver microsomes showed that proteins with molecular weights between 47kDa and 50kDa in liver microsomes were sensitively degraded by lipid peroxidation. Western-blot analysis of liver microsomes with antibodies to P-448-H, P-450 male and P-450 PB-1 which corresponded to P-450d, P-450h and P-450 PCN, respectively, showed that the amount of P-450 PB-1 was decreased more markedly by lipid peroxidation as compared to those of P-450 male and P-448-H. It seemed, therefore, likely that the stability of cytochrome P-450 to lipid peroxidation varies dependent on the forms of cytochrome P-450.
微粒体脂质过氧化作用对乙基吗啡N -脱甲基酶和睾酮6β -羟化酶活性的降低作用，比对苯胺羟化酶、对乙氧基苯胺O -脱乙基酶以及睾酮2α -和16α -羟化酶的降低作用更为显著。肝脏微粒体的十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳结果显示，肝脏微粒体中分子量在47kDa至50kDa之间的蛋白质会因脂质过氧化作用而被敏感降解。用分别对应于P - 450d、P - 450h和P - 450 PCN的抗P - 448 - H、抗P - 450雄性和抗P - 450 PB - 1抗体对肝脏微粒体进行蛋白质免疫印迹分析表明，与P - 450雄性和P - 448 - H相比，脂质过氧化作用使P - 450 PB - 1的量减少得更为显著。因此，细胞色素P - 450对脂质过氧化作用的稳定性可能因细胞色素P - 450的形式不同而有所差异。
